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CD2 antibody | RPA-2.10

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Anti Human CD2 Antibody, clone RPA-2.10 thumbnail image 1
Anti Human CD2 Antibody, clone RPA-2.10 gallery image 1
Figure A. FITC conjugated mouse anti human CD45RA (MCA88F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti human CD45RA (MCA88F) and RPE conjugated mouse anti human CD2 (MCA2814PE). All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).

Mouse anti Human CD2:RPE-Cy5

Product Type
Monoclonal Antibody
Clone
RPA-2.10
Isotype
IgG1
Specificity
CD2

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Product Code Applications Pack Size List Price Your Price Qty
MCA2814C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
loader

Mouse anti Human CD2 antibody, clone RPA-2.10 recognizes the human CD2 cell surface antigen. CD2 is expressed by T lymphocytes and by NK cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
Cynomolgus monkey
Rhesus Monkey
Pig
Baboon
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to RPE-Cy5 - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE-Cy5 488nm laser 496 667
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Acknowledgements
Cy® and CyDye® are registered trademarks of GE Healthcare

Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD2 antibody

  1. Aversa GG et al. (1987) RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation.
    Transplant Proc. 19 (1 Pt 1): 277-8.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Synonyms
LFA-2
RRID
AB_1101170
UniProt
P06729
Entrez Gene
CD2
GO Terms
GO:0001766 membrane raft polarization
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0016337 cell-cell adhesion
GO:0030101 natural killer cell activation
GO:0030887 positive regulation of myeloid dendritic cell activation
GO:0042110 T cell activation
GO:0045580 regulation of T cell differentiation
GO:0050900 leukocyte migration

MCA2814C

161123 167662

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