CD2 antibody | RPA-2.10

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Mouse anti Human CD2

Mouse anti Human CD2:RPE

Mouse anti Human CD2:RPE-Cy5

Product Type
Monoclonal Antibody
Clone
RPA-2.10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2814GA C F 0.1 mg
MCA2814PE F 100 Tests/2ml
MCA2814C F 100 Tests/0.5ml
Mouse anti Human CD2 antibody, clone RPA-2.10 recognizes the human CD2 cell surface antigen. CD2 is expressed by T lymphocytes and by NK cells.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
Cynomolgus monkey
Rhesus Monkey
Pig
Baboon
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to RPE - liquid
Product Form
Purified IgG conjugated to RPE-Cy5 - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.
Shelf Life
6 months from date of despatch.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P06729 Related reagents
Entrez Gene
CD2 Related reagents
GO Terms
GO:0001766 membrane raft polarization
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0016337 cell-cell adhesion
GO:0030101 natural killer cell activation
GO:0030887 positive regulation of myeloid dendritic cell activation
GO:0042110 T cell activation
GO:0045580 regulation of T cell differentiation
GO:0050900 leukocyte migration
Acknowledgements
Cy® and CyDye® are registered trademarks of GE Healthcare
Regulatory
For research purposes only

Applications of CD2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Flow Cytometry Neat
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 20ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD2 antibody

  1. Schernthaner, G.H. et al. (2001) Expression, epitope analysis, and functional role of the LFA-2 antigen detectable on neoplastic mast cells.
    Blood. 98 (13): 3784-92.
  2. Aversa GG et al. (1987) RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation.
    Transplant Proc. 19 (1 Pt 1): 277-8.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.