CD2 antibody | RPA-2.10
Mouse anti Human CD2
Mouse anti Human CD2:RPE
Mouse anti Human CD2:RPE-Cy5
- Product Type
- Monoclonal Antibody
- Clone
- RPA-2.10
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2814GA | C F | 0.1 mg |
|
|
| MCA2814PE | F | 100 Tests/0.5ml |
|
|
| MCA2814C | F | 100 Tests/0.5ml |
|
Mouse anti Human CD2 antibody, clone RPA-2.10 recognizes the human CD2 cell surface antigen. CD2 is expressed by T lymphocytes and by NK cells.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Macaque Cynomolgus monkey Rhesus Monkey Pig Baboon Chimpanzee - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to RPE - liquid
- Product Form
- Purified IgG conjugated to RPE-Cy5 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P06729
- Entrez Gene
- CD2
- GO Terms
- GO:0001766 membrane raft polarization
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006917 induction of apoptosis
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0016337 cell-cell adhesion
- GO:0030101 natural killer cell activation
- GO:0030887 positive regulation of myeloid dendritic cell activation
- GO:0042110 T cell activation
- GO:0045580 regulation of T cell differentiation
- GO:0050900 leukocyte migration
- Acknowledgements
- Cy® and CyDye® are registered trademarks of GE Healthcare
- Regulatory
- For research purposes only
Applications of CD2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/20 | |
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD2 antibody
-
Schernthaner, G.H. et al. (2001) Expression, epitope analysis, and functional role of the LFA-2 antigen detectable on neoplastic mast cells.
Blood. 98 (13): 3784-92. -
Aversa GG et al. (1987) RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation.
Transplant Proc. 19 (1 Pt 1): 277-8.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
