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CD19 antibody | SJ25-C1

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Mouse anti Human CD19:RPE-Cy5

Product Type
Monoclonal Antibody
Clone
SJ25-C1
Isotype
IgG1
Specificity
CD19

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Product Code Applications Pack Size List Price Your Price Qty
MCA1235C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
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Mouse anti Human CD19 antibody, clone SJ25-C1 recognizes the human CD19 cell surface antigen, a 90 kDa glycoprotein present on B lymphocytes.

Target Species
Human
Product Form
Purified IgG conjugated to RPE-Cy5 - liquid
Preparation
Antibody purified from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
sucrose
Immunogen
NALM-1 and NALM-16 cells.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE-Cy5 488nm laser 496 667
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Acknowledgements
Cy and CyDye are registered trademarks of GE Healthcare

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

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References for CD19 antibody

  1. Macallan, D.C. et al. (2005) B-cell kinetics in humans: rapid turnover of peripheral blood memory cells.
    Blood. 105: 3633-40
  2. Alberti, S. et al. (2006) Age-dependent modifications of Type 1 and Type 2 cytokines within virgin and memory CD4+ T cells in humans.
    Mech Ageing Dev. 127: 560-6.
  3. Cox, K. et al. (2005) Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.
    J Leukoc Biol. 78: 1142-52.
  4. Patten, P.E. et al. (2008) CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment.
    Blood. 111: 5173-81.

RRID
AB_321341
UniProt
P15391
Entrez Gene
CD19
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response

MCA1235C

155478 157521 159469 160147 160533 163100 164553 165920

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