CD19 antibody | SJ25-C1

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Mouse anti Human CD19:RPE-Cy5

Product Type
Monoclonal Antibody
Clone
SJ25-C1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1235C F 100 Tests/1ml
Mouse anti Human CD19 antibody, clone SJ25-C1 recognizes the human CD19 cell surface antigen, a 90 kDa glycoprotein present on B lymphocytes.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to RPE-Cy5 - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Sucrose
Immunogen
NALM-1 and NALM-16 cells.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P15391 Related reagents
Entrez Gene
CD19 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0006968 cellular defense response
Acknowledgements
Cy® and CyDye® are registered trademarks of GE Healthcare
Regulatory
For research purposes only

Applications of CD19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD19 antibody

  1. Macallan, D.C. et al. (2005) B-cell kinetics in humans: rapid turnover of peripheral blood memory cells.
    Blood. 105: 3633-40
  2. Alberti, S. et al. (2006) Age-dependent modifications of Type 1 and Type 2 cytokines within virgin and memory CD4+ T cells in humans.
    Mech Ageing Dev. 127: 560-6.
  3. Cox, K. et al. (2005) Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.
    J Leukoc Biol. 78: 1142-52.
  4. Patten, P.E. et al. (2008) CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment.
    Blood. 111: 5173-81.