CD18 antibody | YFC118.3
R-phycoerythrin conjugated Rat anti Human CD18 antibody, clone YFC118.3 (MCA503PE) used for flow cytometry
Image caption:
The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.
From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL.
A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages.
BMC Infect Dis. 2005 May 24;5:39.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD18 antibody, clone YFC118.3 (MCA503) used for immunofluorescence studies using formalin fixed paraffin embedded material following antigen retrieval with citrate buffer at pH6.0
Image caption:
Subretinal MPs accumulate on the retinal pigment epithelium in AMD
A–D Confocal microscopy of IBA‐1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z‐stack projection; (C): oblique Z‐stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E–G Double‐labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA‐1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z‐stack of a subretinal IBA‐1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.
Data information: Controls omitting the primary antibody showed no staining apart from the autofluorescence. AZ: atrophic zone; LD: large drusen. Scale bar: 50 μm (A–D); 10 μm (E–H).
From: Levy O, Calippe B, Lavalette S, Hu SJ, Raoul W, Dominguez E, Housset M, Paques M, Sahel JA, Bemelmans AP, Combadiere C, Guillonneau X, Sennlaub F. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. EMBO Mol Med. 2015 Jan 20. pii: e201404524.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Human CD18 antibody,clone YFC118.3 (MCA503) used for flow cytometry
Image caption:
LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing “high affinity” conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 µg/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.
From: Dilek N, Poirier N, Hulin P, Coulon F, Mary C, et al. (2013)
Targeting CD28, CTLA-4 and PD-L1 Costimulation Differentially Controls Immune Synapses and Function of Human Regulatory and Conventional T-Cells.
PLoS ONE 8(12): e83139.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Phycoerythrin conjugated Rat anti Human CD18 antibody, clone YFC118.3 (MCA503PE) used to identify canine leukocytes by flow cytometry.
Image caption:
Gating strategy for leukocyte identification by means of CD18 expression. Gates for granulocytes and monocytes/lymphocytes were identified using lysed canine whole blood (top panel). CD18 expression relative to isotype control (red population), were documented for granulocytes (middle panel, light blue population) and for monocytes/lymphocytes (bottom panel, blue population). The template was subsequently applied in the assessment of leukocyte contamination of the washed platelets samples. FSC: forward scatter, SSC: side scatter.
From: Cremer SE, Catalfamo JL, Goggs R, Seemann SE, Kristensen AT, Brooks MB (2019)
Proteomic profiling of the thrombin-activated canine platelet secretome (CAPS).
PLoS ONE 14(11): e0224891.
doi:10.1371/journal.pone.0224891
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F IF ResetRat anti Human CD18
- Product Type
- Monoclonal Antibody
- Clone
- YFC118.3
- Isotype
- IgG2b
- Specificity
- CD18
Antibody Quality and Performance Guarantee
| Rat anti Human CD18 antibody, clone YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigens (code number N221) as recognizing the CD18 antigen. CD18 is an integral membrane glycoprotein of ~95 kDa, also known as the beta 2 chain, of the LFA-1 complex. CD18 links non-covalently to either CD11a, b or c molecules forming the heteromeric LFA-1 complex. CD18 acts as the receptor for ICAM-1 and is important for cell adhesion and cell-cell interactions (Reina &Espel 2017). Rat anti Human CD18 antibody, clone YFC118.3 demonstrates strong reactivity with leucocytes and is not reactive with platelets. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Guinea Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human neutrophils
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen 1 | |
1/1000 | 1/5000 |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Human Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2b Negative Control | ||||||
References for CD18 antibody
-
Galfrè, G. et al. (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.
Nature. 277 (5692): 131-3. -
Bindon, C.I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies.
Eur J Immunol. 18 (10): 1507-14. -
Chabanne, L. et al. (1994) Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes.
Tissue Antigens. 43 (3): 202-5. -
Sampaio, W.M. et al. (2007) In vitro binding and survival assays of Leishmania parasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected with Leishmania chagasi.
BMC Vet Res. 3:11. -
Canalli, A.A. et al. (2011) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin.
Haematologica. 96: 526-33. -
Gonçalves, R. et al. (2005) A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages.
BMC Infect Dis. 5:39. -
Zimmerman, K.L. et al. (2013) Leukocyte adhesion deficiency type I in a mixed-breed dog.
J Vet Diagn Invest. 25: 291-6. -
Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75.
View The Latest Product References
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Garland, R.J. et al. (2002) Human CD8+ CTL recognition and in vitro lysis of herpes simplex virus-infected cells by a non-MHC restricted mechanism.
Scand J Immunol. 55: 61-9. -
Grote, K. et al. (2007) The angiogenic factor CCN1 promotes adhesion and migration of circulating CD34+ progenitor cells: potential role in angiogenesis and endothelial regeneration.
Blood. 110: 877-85. -
Holst, B.S. et al. (2010) Expression of four canine leukocyte adhesion factors in fresh and stored whole blood samples evaluated using a no-lyse, no-wash method.
Vet Immunol Immunopathol. 139: 271-6. -
Kupatt, C. et al. (1999) Tumor necrosis factor-alpha contributes to ischemia- and reperfusion-induced endothelial activation in isolated hearts.
Circ Res. 84: 392-400. -
Marconato, L. (2013) The dog as a possible animal model for human non-Hodgkin lymphoma: a review.
Hematol Oncol. 31: 1-9. -
Spring, F.A. et al. (2001) Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms.
Blood. 98: 458-66. -
Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
Circulation. 104: 2975-80. -
Mizuno, T. et al. (1997) cDNA cloning and chromosomal localization of the human telencephalin and its distinctive interaction with lymphocyte function-associated antigen-1.
J Biol Chem. 272: 1156-63. -
Lana, S. et al. (2006) Diagnosis of mediastinal masses in dogs by flow cytometry.
J Vet Intern Med. 20: 1161-5. -
Waché, Y.J. et al. (2009) The mycotoxin deoxynivalenol inhibits the cell surface expression of activation markers in human macrophages.
Toxicology. 262: 239-44. -
Crosby, H.A. et al. (2009) Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
J Hepatol. 51: 734-49. -
Levy, O. et al. (2015) Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
EMBO Mol Med. pii: e201404524. -
Alex, J. et al. (2005) Pretreatment with hyperbaric oxygen and its effect on neuropsychometric dysfunction and systemic inflammatory response after cardiopulmonary bypass: a prospective randomized double-blind trial.
J Thorac Cardiovasc Surg. 130: 1623-30. -
Sutcliffe, J.E.S. et al. (2017) Changes in the extracellular matrix surrounding human chronic wounds revealed by 2-photon imaging.
Int Wound J. Jul 20 [Epub ahead of print]. -
Cremer, S.E. et al. (2019) Proteomic profiling of the thrombin-activated canine platelet secretome (CAPS).
PLoS One. 14 (11): e0224891. -
Wolf-Ringwall, A. et al. (2020) Prospective evaluation of flow cytometric characteristics, histopathologic diagnosis and clinical outcome in dogs with naïve B-cell lymphoma treated with a 19-week CHOP protocol.
Vet Comp Oncol. 18 (3): 342-52.
- Synonyms
- Integrin Beta 2 Chain
- RRID
- AB_321325
- UniProt
- P05107
- Entrez Gene
- ITGB2
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0007596 blood coagulation
- GO:0006915 apoptosis
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0006954 inflammatory response
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- View More GO Terms
- GO:0007267 cell-cell signaling
- GO:0007275 multicellular organismal development
- GO:0008360 regulation of cell shape
- GO:0019901 protein kinase binding
- GO:0030593 neutrophil chemotaxis
- GO:0050730 regulation of peptidyl-tyrosine phosphorylation
- GO:0050776 regulation of immune response
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