CD18 antibody | YFC118.3

100% Secure


Rat anti Human CD18:Alexa Fluor® 647

Rat anti Human CD18:FITC

Rat anti Human CD18:Low Endotoxin

Rat anti Human CD18:Pacific Blue®

Rat anti Human CD18

Rat anti Human CD18:RPE

Product Type
Monoclonal Antibody
Clone
YFC118.3
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA503A647T F 25 Tests/0.25ml
MCA503A647 F 100 Tests/1ml
MCA503F F 100 Tests
MCA503EL C* F FN IF IP 0.5 mg
MCA503PB F 100 Tests/1ml
MCA503GA C* F IF IP 0.1 mg
MCA503 C* F IF IP 0.2 mg
MCA503PET F 25 Tests
MCA503PE F 100 Tests
Rat anti Human CD18 antibody, clone YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen (code number N221) as recognising the CD18 antigen.

The CD18 antigen is an integral membrane glycoprotein of ~95 kDa, known as the beta chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules.

The main cellular reactivity of Rat anti Human CD18 antibody, clone YFC118.3 is a strong reactivity with Leucocytes (platelets negative).

The CD18 molecule is the receptor for ICAM-1 and is thus important for cell adhesion.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Human neutrophils.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of reconstitution.

More Information

UniProt
P05107 Related reagents
Entrez Gene
ITGB2 Related reagents
GO Terms
GO:0001948 glycoprotein binding
GO:0007596 blood coagulation
GO:0006915 apoptosis
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007267 cell-cell signaling
GO:0007275 multicellular organismal development
GO:0008360 regulation of cell shape
GO:0019901 protein kinase binding
GO:0030593 neutrophil chemotaxis
GO:0050730 regulation of peptidyl-tyrosine phosphorylation
GO:0050776 regulation of immune response
Acknowledgements
The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.

This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.
Acknowledgements
The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.
Regulatory
For research purposes only

Applications of CD18 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Functional Assays
Immunofluorescence
Immunohistology - Frozen 1 1/1000 1/5000
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1 1/1000 1/5000
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry Neat
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul whole blood or 106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil
Histology Positive Control Tissue
Human Tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2b Negative Control:Alexa Fluor® 647 MCA6006A647 100 Tests/1ml F
Rat IgG2b Negative Control:FITC MCA6006F 0.1 mg F
Rat IgG2b Negative Control:Low Endotoxin MCA6006EL 0.5 mg F
Rat IgG2b Negative Control:Pacific Blue® MCA6006PB 100 Tests/1ml F
Rat IgG2b Negative Control MCA6006GA 0.1 mg F
Rat IgG2b Negative Control:RPE MCA6006PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for CD18 antibody

  1. Bindon, C.I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies.
    Eur J Immunol. 18 (10): 1507-14.
  2. Chabanne, L. et al. (1994) Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes.
    Tissue Antigens. 43 (3): 202-5.
  3. Sampaio, W.M. et al. (2007) In vitro binding and survival assays of Leishmania parasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected with Leishmania chagasi.
    BMC Vet Res. 3:11.
  4. Canalli, A.A. et al. (2011) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin.
    Haematologica. 96: 526-33.
  5. Gonçalves, R. et al. (2005) A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages.
    BMC Infect Dis. 5:39.
  6. Zimmerman, K.L. et al. (2013) Leukocyte adhesion deficiency type I in a mixed-breed dog.
    J Vet Diagn Invest. 25: 291-6.
  7. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
    Vet Immunol Immunopathol. 141: 64-75.
  8. Garland, R.J. et al. (2002) Human CD8+ CTL recognition and in vitro lysis of herpes simplex virus-infected cells by a non-MHC restricted mechanism.
    Scand J Immunol. 55: 61-9.
  9. Grote, K. et al. (2007) The angiogenic factor CCN1 promotes adhesion and migration of circulating CD34+ progenitor cells: potential role in angiogenesis and endothelial regeneration.
    Blood. 110: 877-85.
  10. Holst, B.S. et al. (2010) Expression of four canine leukocyte adhesion factors in fresh and stored whole blood samples evaluated using a no-lyse, no-wash method.
    Vet Immunol Immunopathol. 139: 271-6.
  11. Kupatt, C. et al. (1999) Tumor necrosis factor-alpha contributes to ischemia- and reperfusion-induced endothelial activation in isolated hearts.
    Circ Res. 84: 392-400.
  12. Marconato, L. (2013) The dog as a possible animal model for human non-Hodgkin lymphoma: a review.
    Hematol Oncol. 31: 1-9.
  13. Spring, F.A. et al. (2001) Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms.
    Blood. 98: 458-66.
  14. Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
    Circulation. 104: 2975-80.
  15. Mizuno, T. et al. (1997) cDNA cloning and chromosomal localization of the human telencephalin and its distinctive interaction with lymphocyte function-associated antigen-1.
    J Biol Chem. 272: 1156-63.
  16. Lana, S. et al. (2006) Diagnosis of mediastinal masses in dogs by flow cytometry.
    J Vet Intern Med. 20: 1161-5.
  17. Waché, Y.J. et al. (2009) The mycotoxin deoxynivalenol inhibits the cell surface expression of activation markers in human macrophages.
    Toxicology. 262: 239-44.
  18. Crosby, H.A. et al. (2009) Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    J Hepatol. 51: 734-49.
  19. Levy, O. et al. (2015) Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
    EMBO Mol Med. pii: e201404524.
  20. Alex, J. et al. (2005) Pretreatment with hyperbaric oxygen and its effect on neuropsychometric dysfunction and systemic inflammatory response after cardiopulmonary bypass: a prospective randomized double-blind trial.
    J Thorac Cardiovasc Surg. 130: 1623-30.
  21. Sutcliffe, J.E.S. et al. (2017) Changes in the extracellular matrix surrounding human chronic wounds revealed by 2-photon imaging.
    Int Wound J. Jul 20 [Epub ahead of print].