CD18 antibody | YFC118.3








Rat anti Human CD18:Alexa Fluor® 647
Rat anti Human CD18:FITC
Rat anti Human CD18:Low Endotoxin
Rat anti Human CD18:Pacific Blue®
Rat anti Human CD18
Rat anti Human CD18:RPE
Rat anti Human CD18:Alexa Fluor® 700
Rat anti Human CD18:Amethyst Orange
- Product Type
- Monoclonal Antibody
- Clone
- YFC118.3
- Isotype
- IgG2b
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA503A647T | F | 25 Tests/0.25ml |
![]() |
|
MCA503A647 | F | 100 Tests/1ml |
![]() |
|
MCA503F | F | 100 Tests |
![]() |
|
MCA503EL | C* F FN IF IP | 0.5 mg |
![]() |
|
MCA503PB | F | 100 Tests/1ml |
![]() |
|
MCA503GA | C* F IF IP | 0.1 mg |
![]() |
|
MCA503 | C* F IF IP | 0.2 mg |
![]() |
|
MCA503PE | F | 100 Tests |
![]() |
|
MCA503A700 | F | 100 Tests/1ml |
![]() |
|
MCA503AMO | F | 0.1 mg |
![]() |
CD18 is an integral membrane glycoprotein of ~95 kDa, also known as the beta 2 chain, of the LFA-1 complex. CD18 links non-covalently to either CD11a, b or c molecules forming the heteromeric LFA-1 complex. CD18 acts as the receptor for ICAM-1 and is important for cell adhesion and cell-cell interactions (Reina &Espel 2017).
Rat anti Human CD18 antibody, clone YFC118.3 demonstrates strong reactivity with leucocytes and is not reactive with platelets.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Guinea Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor 700 - liquid
- Product Form
- Purified IgG conjugated to Amethyst Orange - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Human neutrophils
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of reconstitution.
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05107 Related reagents
- Entrez Gene
- ITGB2 Related reagents
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0007596 blood coagulation
- GO:0006915 apoptosis
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0006954 inflammatory response
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007267 cell-cell signaling
- GO:0007275 multicellular organismal development
- GO:0008360 regulation of cell shape
- GO:0019901 protein kinase binding
- GO:0030593 neutrophil chemotaxis
- GO:0050730 regulation of peptidyl-tyrosine phosphorylation
- GO:0050776 regulation of immune response
- Acknowledgements
- The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com - Acknowledgements
- The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.
- Acknowledgements
- The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.
- Acknowledgements
- The isolation and characterisation of this cell line was carried out with help of funds supplied by the Medical Research Council, UK.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com - Regulatory
- For research purposes only
Applications of CD18 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Flow Cytometry | 1/50 | 1/100 | |
Functional Assays | |||
Immunofluorescence | |||
Immunohistology - Frozen 1 | 1/1000 | 1/5000 | |
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Flow Cytometry | Neat | ||
Flow Cytometry | 1/50 | 1/100 | |
Immunofluorescence | |||
Immunohistology - Frozen 1 | 1/1000 | 1/5000 | |
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | 1/2 | |
Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 100ul whole blood or 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Histology Positive Control Tissue
- Human tonsil
- Histology Positive Control Tissue
- Human Tonsil
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2b Negative Control:Alexa Fluor® 647 | MCA6006A647 | F | 100 Tests/1ml |
![]() |
|
Rat IgG2b Negative Control:FITC | MCA6006F | F | 0.1 mg |
![]() |
|
Rat IgG2b Negative Control:Low Endotoxin | MCA6006EL | F | 0.5 mg |
![]() |
|
Rat IgG2b Negative Control:Pacific Blue® | MCA6006PB | F | 100 Tests/1ml |
![]() |
|
Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
![]() |
|
Rat IgG2b Negative Control:RPE | MCA6006PE | F | 100 Tests |
![]() |
|
Rat IgG2b Negative Control:Alexa Fluor® 700 | MCA6006A700 | F | 100 Tests/1ml |
![]() |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD18 antibody
-
Bindon, C.I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies.
Eur J Immunol. 18 (10): 1507-14. -
Chabanne, L. et al. (1994) Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes.
Tissue Antigens. 43 (3): 202-5. -
Sampaio, W.M. et al. (2007) In vitro binding and survival assays of Leishmania parasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected with Leishmania chagasi.
BMC Vet Res. 3:11. -
Canalli, A.A. et al. (2011) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin.
Haematologica. 96: 526-33. -
Gonçalves, R. et al. (2005) A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages.
BMC Infect Dis. 5:39. -
Zimmerman, K.L. et al. (2013) Leukocyte adhesion deficiency type I in a mixed-breed dog.
J Vet Diagn Invest. 25: 291-6. -
Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. -
Garland, R.J. et al. (2002) Human CD8+ CTL recognition and in vitro lysis of herpes simplex virus-infected cells by a non-MHC restricted mechanism.
Scand J Immunol. 55: 61-9. -
Grote, K. et al. (2007) The angiogenic factor CCN1 promotes adhesion and migration of circulating CD34+ progenitor cells: potential role in angiogenesis and endothelial regeneration.
Blood. 110: 877-85. -
Holst, B.S. et al. (2010) Expression of four canine leukocyte adhesion factors in fresh and stored whole blood samples evaluated using a no-lyse, no-wash method.
Vet Immunol Immunopathol. 139: 271-6. -
Kupatt, C. et al. (1999) Tumor necrosis factor-alpha contributes to ischemia- and reperfusion-induced endothelial activation in isolated hearts.
Circ Res. 84: 392-400. -
Marconato, L. (2013) The dog as a possible animal model for human non-Hodgkin lymphoma: a review.
Hematol Oncol. 31: 1-9. -
Spring, F.A. et al. (2001) Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms.
Blood. 98: 458-66. -
Salvatierra, A. et al. (2001) Antithrombin III prevents early pulmonary dysfunction after lung transplantation in the dog.
Circulation. 104: 2975-80. -
Mizuno, T. et al. (1997) cDNA cloning and chromosomal localization of the human telencephalin and its distinctive interaction with lymphocyte function-associated antigen-1.
J Biol Chem. 272: 1156-63. -
Lana, S. et al. (2006) Diagnosis of mediastinal masses in dogs by flow cytometry.
J Vet Intern Med. 20: 1161-5. -
Waché, Y.J. et al. (2009) The mycotoxin deoxynivalenol inhibits the cell surface expression of activation markers in human macrophages.
Toxicology. 262: 239-44. -
Crosby, H.A. et al. (2009) Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
J Hepatol. 51: 734-49. -
Levy, O. et al. (2015) Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
EMBO Mol Med. pii: e201404524. -
Alex, J. et al. (2005) Pretreatment with hyperbaric oxygen and its effect on neuropsychometric dysfunction and systemic inflammatory response after cardiopulmonary bypass: a prospective randomized double-blind trial.
J Thorac Cardiovasc Surg. 130: 1623-30. -
Sutcliffe, J.E.S. et al. (2017) Changes in the extracellular matrix surrounding human chronic wounds revealed by 2-photon imaging.
Int Wound J. Jul 20 [Epub ahead of print]. -
Cremer, S.E. et al. (2019) Proteomic profiling of the thrombin-activated canine platelet secretome (CAPS).
PLoS One. 14 (11): e0224891.