CD160 antibody | BY55

100% Secure


Mouse anti Human CD160:Preservative Free

Mouse anti Human CD160:FITC

Mouse anti Human CD160

Product Type
Monoclonal Antibody
Clone
BY55
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2238XZ F IP 1 mg
MCA2238F F 0.1 mg
MCA2238 F IP 0.2 mg
Mouse anti Human CD160 antibody, clone BY55 recognizes human CD160, a ~27 kDa cell surface glycoprotein that was initially identified by clone BY55. The expression of CD160 is restricted to circulating NK cells, lymphocytes expressing T-cell receptor (TCR) gamma/delta and a small population of TCR alpha/beta positive T cells.

CD160 is expressed by intestinal intraepithelial lymphocytes.

Product Details

Target Species
Human
Product Form
Purified IgM - liquid
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared from tissue culture supernatant
Preparation
Purified IgM prepared from tissue culture supernatant
Preparation
Purified IgM prepared from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human NK cell line YT2C2.
Approx. Protein Concentrations
IgM concentration 1.0 mg/ml
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma.

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O95971 Related reagents
Entrez Gene
CD160 Related reagents
GO Terms
GO:0005102 receptor binding
GO:0046658 anchored to plasma membrane
GO:0006968 cellular defense response
GO:0007166 cell surface receptor linked signaling pathway
GO:0008283 cell proliferation
GO:0032393 MHC class I receptor activity
GO:0050776 regulation of immune response
Regulatory
For research purposes only

Applications of CD160 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/50
Immunoprecipitation
Flow Cytometry
Flow Cytometry 1/10 1/50
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgM:FITC 102002 1 mg F
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgM Negative Control MCA692 100 Tests F
Mouse IgM Negative Control:FITC MCA692F 0.1 mg F
Mouse IgM Negative Control MCA692 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD160 antibody

  1. Anumanthan, A. et al. (1998) Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes.
    J Immunol. 161 (6): 2780-90.
  2. Maiza, H. et al. (1993) A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity.
    J Exp Med. 178: 1121-6.
  3. Nikolova, M. et al. (2002) BY55/CD160 acts as a co-receptor in TCR signal transduction of a human circulating cytotoxic effector T lymphocyte subset lacking CD28 expression.
    Int Immunol. 14 (5): 445-51.