CD160 antibody | BY55

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Mouse anti Human CD160

Product Type
Monoclonal Antibody
Clone
BY55
Isotype
IgM
Product Code Applications Pack Size List Price Quantity
MCA2238
Datasheet
F IP
SDS
0.2 mg loader

Mouse anti Human CD160 antibody, clone BY55 recognizes human CD160, a ~27 kDa cell surface glycoprotein that was initially identified by clone BY55. The expression of CD160 is restricted to circulating NK cells, lymphocytes expressing T-cell receptor (TCR) gamma/delta and a small population of TCR alpha/beta positive T cells.

CD160 is expressed by intestinal intraepithelial lymphocytes.

Product Details

Target Species
Human
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Human NK cell line YT2C2.
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
O95971
Entrez Gene
CD160
GO Terms
GO:0005102 receptor binding
GO:0046658 anchored to plasma membrane
GO:0006968 cellular defense response
GO:0007166 cell surface receptor linked signaling pathway
GO:0008283 cell proliferation
GO:0032393 MHC class I receptor activity
GO:0050776 regulation of immune response
Regulatory
For research purposes only

Applications of CD160 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgM:FITC HCA040F F 0.1 mg loader
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A C E P WB 1 ml loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgM Negative Control MCA692 F 100 Tests loader

Product Specific References

Source Reference

  1. Maiza, H. et al. (1993) A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity.
    J Exp Med. 178: 1121-6.

References for CD160 antibody

  1. Anumanthan, A. et al. (1998) Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes.
    J Immunol. 161 (6): 2780-90.
  2. Nikolova, M. et al. (2002) BY55/CD160 acts as a co-receptor in TCR signal transduction of a human circulating cytotoxic effector T lymphocyte subset lacking CD28 expression.
    Int Immunol. 14 (5): 445-51.

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