Beclin 1 antibody | OTI3F3
Mouse anti Beclin 1
Beclin 1 antibody recognizes beclin 1, also known as ATG6 autophagy related 6 homolog, or coiled-coil myosin-like BCL2-interacting protein.
Beclin 1 Antibody with Control Lysate
Beclin 1 antibody recognizes beclin 1, also known as ATG6 autophagy related 6 homolog, or coiled-coil myosin-like BCL2-interacting protein.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3F3
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00721 | WB | 100 µl | ||||
| VMA00721KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human beclin 1 antibody recognizes beclin 1, also known as ATG6 autophagy related 6 homolog and coiled-coil myosin-like Bcl2-interacting protein.
Beclin 1 participates in the regulation of autophagy and has an important role in development, tumorigenesis and neurodegeneration (Zhong et al., 2009 (PubMed 19270693)) (supplied by OMIM, Jul 2010).
Mouse anti Human beclin 1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Beclin 1 participates in the regulation of autophagy and has an important role in development, tumorigenesis and neurodegeneration (Zhong et al., 2009 (PubMed 19270693)) (supplied by OMIM, Jul 2010).
Mouse anti Human beclin 1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti beclin 1 detects a band of approximately 60 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Immunogen
- HEK293-derived full length recombinant protein of human beclin 1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q14457 Related reagents
- Entrez Gene
- BECN1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0009615 response to virus
- GO:0006916 anti-apoptosis
- GO:0006968 cellular defense response
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Beclin 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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