AKR1C2 antibody

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Anti AKR1C2 Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti AKR1C2 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with AKR1C2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2.79 second exposure. Arrow points to AKR1C2 (molecular weight 36 kDa)

Anti AKR1C2 Antibody (PrecisionAb Monoclonal Antibody) gallery image 2 — Figure A. FITC conjugated mouse anti human CD95 (MCA1539F) and Mouse anti mouse IgG2a isotype control (MCA929) detected with RPE conjugated goat anti mouse IgG2a (STAR133PE). Figure B. FITC conjugated mouse anti human CD95 (MCA1539F) and Mouse anti AKR1C2 (VMA00346) detected with RPE conjugated goat anti mouse IgG2a (STAR133PE). All experiments performed on Leucoperm (BUF09) fixed and permeabilized HeLa cells gated on live single cells. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti AKR1C2

Product Type: PrecisionAb Monoclonal
Isotype: IgG2a
Format: Purified
Specificity: AKR1C2

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00346 Datasheet Datasheet SDS SDS	F WB	100 µl		Log in	Get Quote
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Mouse anti Human AKR1C2 antibody recognizes the aldo-keto reductase family 1 member C2, also known as 3-alpha-HSD3, DD-2, DD/BABP, aldo-keto reductase family 1 member C2, chlordecone reductase homolog HAKRD, dihydrodiol dehydrogenase 2, bile acid binding protein, 3-alpha hydroxysteroid dehydrogenase, type III, pseudo-chlordecone reductase, testicular 17,20-desmolase deficiency, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase and type II dihydrodiol dehydrogenase.

Encoded by the AKR1C2 gene, aldo-keto reductase family 1 member C2 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. AKR1C2 shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for AKR1C2 (provided by RefSeq, Dec 2011).

Mouse anti Human AKR1C2 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti AKR1C2 detects a band of approximately 36 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant human AKR1C2
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P52895
Entrez Gene: AKR1C2
GO Terms: GO:0005737 cytoplasm; GO:0055114 oxidation-reduction process; GO:0006693 prostaglandin metabolic process; GO:0007586 digestion; GO:0008202 steroid metabolic process; GO:0032052 bile acid binding; GO:0047026 androsterone dehydrogenase (A-specific) activity; GO:0047115 trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity