14-3-3 Zeta/Delta antibody

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Mouse anti 14-3-3 Zeta/Delta

14-3-3 Zeta/Delta antibody recognizes the 14-3-3 protein zeta/delta, also known as 14-3-3 delta, 14-3-3 protein/cytosolic phospholipase A2, 14-3-3 zeta, epididymis luminal protein 4, epididymis secretory protein Li 3, or phospholipase A2.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00299
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human 14-3-3 zeta/delta antibody recognizes the 14-3-3 protein zeta/delta also known as 14-3-3 delta, 14-3-3 protein/cytosolic phospholipase A2, 14-3-3 zeta, epididymis luminal protein 4, epididymis secretory protein Li 3, phospholipase A2, protein kinase C inhibitor protein 1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide and tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide.

A product of the YWHAZ gene, 14-3-3 zeta/delta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for YWHAZ (provided by RefSeq, Oct 2008).

Mouse anti Human 14-3-3 protein zeta/delta antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti 14-3-3 zeta/delta detects a band of approximately 28 kDa in A431 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein from human 14-3-3 zeta/delta
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P63104 Related reagents
Entrez Gene
YWHAZ Related reagents
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of 14-3-3 Zeta/Delta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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