14-3-3 Beta/Alpha antibody

100% Secure


Mouse anti 14-3-3 Beta/Alpha

14-3-3 beta/alpha antibody recognizes 14-3-3 protein beta/alpha, also known as 14-3-3 alpha, 14-3-3 protein beta/alpha, brain protein 14-3-3, beta isoform, epididymis secretory protein Li 1, protein 1054, or protein kinase C inhibitor protein 1.

14-3-3 Beta/Alpha Antibody with Control Lysate

14-3-3 beta/alpha antibody recognizes 14-3-3 protein beta/alpha, also known as 14-3-3 alpha, 14-3-3 protein beta/alpha, brain protein 14-3-3, beta isoform, epididymis secretory protein Li 1, protein 1054, or protein kinase C inhibitor protein 1.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00333 WB 100 µl
VMA00333KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human 14-3-3 beta/alpha antibody recognizes the 14-3-3 protein beta/alpha, also known as 14-3-3 alpha, brain protein 14-3-3, beta isoform, epididymis secretory protein Li 1, protein 1054, protein kinase C inhibitor protein 1, protein kinase C inhibitor protein-1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide.

Encoded by the YWHAB gene, 14-3-3 protein beta/alpha belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for YWHAB (provided by RefSeq, Jul 2008).

Mouse anti Human 14-3-3 protein beta/alpha antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti 14-3-3 beta/alpha detects a band of approximately 28 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human 14-3-3 beta/alpha
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P31946 Related reagents
Entrez Gene
YWHAB Related reagents
GO Terms
GO:0000186 activation of MAPKK activity
GO:0005829 cytosol
GO:0005813 centrosome
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008286 insulin receptor signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0016071 mRNA metabolic process
GO:0019904 protein domain specific binding
GO:0035308 negative regulation of protein dephosphorylation
GO:0042470 melanosome
GO:0042826 histone deacetylase binding
GO:0050815 phosphoserine binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0048471 perinuclear region of cytoplasm
GO:0051220 cytoplasmic sequestering of protein
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of 14-3-3 Beta/Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted