Guselkumab antibody | AbD59938ia
Human anti Guselkumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD59938ia
- Isotype
- IgG1
- Specificity
- Guselkumab
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human anti guselkumab antibody, clone AbD59938ia (HCA420) at a concentration of 2μg/mL in HISPEC assay diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate.
HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with guselkumab, human IgG1 kappa control, human IgG1 lambda control or human recombinant IL-23A at a concentration of 5μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human anti guselkumab antibody, clone AbD59938ia (HCA420) titrated to the given concentrations in HISPEC assay diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate.
Data for guselkumab are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with recombinant IL-23A at a concentration of 1μg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of guselkumab (0.15μg/ml) plus increasing concentrations of Human anti guselkumab antibody, clone AbD59938ia (HCA420) in the monovalent Fab format was added. Free guselkumab, still capable of binding to the IL-23A coated plate was detected using HRP conjugated Mouse anti Human IgG (Fc) CH2 domain antibody, clone MK 1 A6 (MCA647P) followed by QuantaBlu fluorogenic peroxidase substrate.
Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with guselkumab at a concentration of 1%mu;g/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human anti guselkumab antibody, clone AbD59938ia (HCA420, black) or clone AbD59947ia (HCA421, red). Detection was performed using HRP conjugated guselkumab at a concentration of 2μg/ml in HISPEC assay diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate.
Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
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E Reset| Human Anti-Guselkumab Antibody, clone AbD59938ia is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug guselkumab, and its biosimilar products. It does not recognize the drug target, the human Interleukin-23 (IL-23), nor guselkumab in complex with IL-23. HCA420 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of <0.06nM for guselkumab and is one of the two antibodies offered for ADA assays. Antibody HCA421 has an affinity of 0.3nM for guselkumab. Guselkumab (trade name Tremfya) is a fully human monoclonal antibody of the IgG1/lambda isotype binding to the p19 subunit of IL-23 to block its interaction with the IL-23 receptor, subsequently inhibiting the release of pro-inflammatory cytokines and chemokines. Guselkumab is approved for the treatment of moderate to severe plaque psoriasis in adults. View a summary of all anti-guselkumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This product is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Guselkumab
- Affinity
- The monovalent intrinsic affinity of Human Anti-Guselkumab Antibody, clone AbD59938ia was measured as KD = 0.06 nM by real time, label-free molecular interaction analysis on immobilized guselkumab
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Tremfya is a trademark of MorphoSys - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Clone AbD59938ia can be used in indirect ELISA to detect guselkumab. It can also be used as a surrogate positive control or a calibrator in an anti-drug antibody (ADA) assay, together with HCA421
Protocol: ADA bridging ELISA
- Synonyms
- Tremfya
HCA420
169955
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Guselkumab specificity
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