Guselkumab antibody | AbD59967
Human anti Guselkumab (Drug/Target Complex):HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD59967
- Isotype
- Fab antibody
- Specificity
- Guselkumab
- Region
- DRUG/TARGET COMPLEX
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5μg/ml. After washing and blocking with PBST + 5% BSA, the IL-23A/guselkumab complex was formed by adding 5μg/mL guselkumab to the wells coated with 5μg/mL IL-23A. Detection was performed using Human anti guselkumab (Drug/Target Complex) Antibody, clone AbD59967ad (TZA072) at a concentration of 2μg/ml followed by HRP conjugated anti DYKDDDDK tag antibody in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with guselkumab, human IgG1 kappa control, human IgG1 lambda control, normal human serum (NHS) or human recombinant IL-23A at a concentration of 5μg/ml. After washing and blocking with PBST + 5% BSA, the IL-23A/guselkumab complex was formed by adding 5μg/mL guselkumab to the wells coated with 5μg/mL IL-23A. Detection was performed using Human anti guselkumab (drug/target complex) antibody, clone AbD59967ad (TZA072) titrated to the given concentrations followed by an HRP conjugated anti DYKDDDDK tag antibody in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
Data for IL-23A/guselkumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with IL-23A at a concentration of 5μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of guselkumab. Detection was performed using HRP conjugated Human anti guselkumab (drug/target complex) antibody, clone AbD59967pap (TZA072P) at a concentration of 0.5μg/mL in HISPEC assay diluent (BUF049A), and QuantaBlu fluorogenic peroxidase substrate.
Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human anti guselkumab (drug/target complex) Antibody, clone AbD59967 recognizes the antibody drug guselkumab when in complex with its target, the human Interleukin-23 (IL-23). It does not recognize free guselkumab nor unbound IL-23. Clone AbD59967 can be used to develop a pharmacokinetic (PK) antigen capture assay to measure levels of guselkumab captured via immobilized IL-23. TZA072 is a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog. Clone AbD59967 is also available conjugated to HRP via coupling to the BiSpyCatcher2:HRP (TZC002P) for direct detection of the IL-23/guselkumab complex. Guselkumab (trade name Tremfya) is a fully human monoclonal antibody of the IgG1/lambda isotype binding to the p19 subunit of IL-23 to block its interaction with the IL-23 receptor, subsequently inhibiting the release of pro-inflammatory cytokines and chemokines. Guselkumab is approved for the treatment of moderate to severe plaque psoriasis in adults. View a summary of all anti-guselkumab antibodies |
- Product Form
- A bivalent human recombinant Fab (kappa light chain) generated by coupling of the monovalent SpyTagged Fab TZA072 with a HRP conjugated BiSpyCatcher version 2 (TZC002P). The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Guselkumab
- Affinity
- The monovalent intrinsic affinity of AbD59967pap was measured as KD = 33 nM by real time, label free molecular interaction analysis on immobilized IL-23A/guselkumab complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Tremfya is a trademark of MorphoSys. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD59967pap can be used in direct ELISA to detect the IL-23A/ guselkumab complex in a pharmacokinetic (PK) antigen capture assay.
Protocol: PK antigen capture ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Guselkumab | HCA420 | E | 0.1 mg |
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| Description | Human anti Guselkumab | ||||||
| Human anti Guselkumab | HCA421 | E | 0.1 mg |
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| Description | Human anti Guselkumab | ||||||
| Human anti Guselkumab | TZA070 | E | 0.1 mg |
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| Description | Human anti Guselkumab | ||||||
| Human anti Guselkumab | TZA071 | E | 0.1 mg |
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| Description | Human anti Guselkumab | ||||||
| Human anti Guselkumab:HRP | TZA071P | E | 0.1 mg |
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| Description | Human anti Guselkumab:HRP | ||||||
| Human anti Guselkumab (Drug/Target Complex) | TZA072 | E | 0.1 mg |
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| Description | Human anti Guselkumab (Drug/Target Complex) | ||||||
- Synonyms
- Tremfya
TZA072P
169961
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Guselkumab specificity
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