Macrophages/Monocytes antibody | MR-1

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Mouse anti Guinea Pig Macrophages

Product Type
Monoclonal Antibody
Clone
MR-1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA518S C F* 2 ml
Mouse anti Guinea Pig macrophages / monocytes antibody, clone MR-1 recognizes a cytoplasmic component of guinea pig monocytes and most tissue macrophages. Mouse anti Guinea Pig macrophages / monocytes antibody, clone MR-1 does not recognise cortical macrophages of the thymus or microglial cells of the brain.

Product Details

Target Species
Guinea Pig
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 5-10% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Guinea Pig lymph node stroma.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Macrophages/Monocytes antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Immunohistology - Frozen 1/10
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for Macrophages/Monocytes antibody

  1. Kraal, G. et al. (1988) Histochemical identification of guinea-pig macrophages by monoclonal antibody MR-1.
    Immunology. 65 (4): 523-8.
  2. Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
    Antimicrob Agents Chemother. 55 (1): 124-31.
  3. Mateo, M. et al. (2011) VP24 is a molecular determinant of Ebola virus virulence in guinea pigs.
    J Infect Dis. 204 Suppl 3: S1011-20.
  4. Bendig, M.M. et al. (2007) Humanized antibodies against leukocyte adhesion molecule VLA-4
    Patent Appl WO 1995019790 A1