Macrophages/Monocytes antibody | MR-1

Mouse anti Guinea Pig Macrophages

Product Type
Monoclonal Antibody
Clone
MR-1
Isotype
IgG1
Specificity
Macrophages/Monocytes

Product Code Applications Pack Size List Price Your Price Qty
MCA518S
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C F * 2 ml loader
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Mouse anti Guinea Pig macrophages / monocytes antibody, clone MR-1 recognizes a cytoplasmic component of guinea pig monocytes and most tissue macrophages. Mouse anti Guinea Pig macrophages / monocytes antibody, clone MR-1 does not recognise cortical macrophages of the thymus or microglial cells of the brain.

Target Species
Guinea Pig
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 2% foetal bovine serum.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3).
Immunogen
Guinea Pig lymph node stroma.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Immunohistology - Frozen 1/10
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for Macrophages/Monocytes antibody

  1. Kraal, G. et al. (1988) Histochemical identification of guinea-pig macrophages by monoclonal antibody MR-1.
    Immunology. 65 (4): 523-8.
  2. Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs.
    Antimicrob Agents Chemother. 55 (1): 124-31.
  3. Mateo, M. et al. (2011) VP24 is a molecular determinant of Ebola virus virulence in guinea pigs.
    J Infect Dis. 204 Suppl 3: S1011-20.
  4. Bendig, M.M. et al. (2007) Humanized antibodies against leukocyte adhesion molecule VLA-4
    Patent Appl WO 1995019790 A1

RRID
AB_322840