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Golimumab antibody | AbD20710

Human anti Golimumab

Product Type
Monoclonal Antibody
Clone
AbD20710
Isotype
HuCAL Fab monovalent
Specificity
Golimumab

Product Code Applications Pack Size List Price Your Price Qty
HCA240
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader

Human anti golimumab antibody, clone AbD20710 is a Type 1 anti-idiotypic antibody that recognizes free golimumab but not the drug/tumor necrosis factor alpha (TNFα) complex. Clone AbD20710 inhibits the binding of golimumab to TNFα and can be used to measure free golimumab levels in serum from patients, or for pharmacokinetic (PK) assay development.

An affinity matured variant of HCA240 is available with about 2-fold affinity improvement, clone AbD25429 (HCA286).

Golimumab, marketed under the brand name Simponi, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

View a summary of all anti-golimumab antibodies

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography.
Source
E.coli
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Golimumab.
Affinity
The intrinsic affinity of the monovalent form of Human anti Golimumab antibody, clone AbD20710 is KD = 2.3 nM as measured by real time, label free molecular interaction analysis on immobilized Golimumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Simponi is a trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
His-tag is a trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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Synonyms
Simponi
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA240

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View more products with GOLIMUMAB specificity

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