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Etanercept antibody | AbD25940_hIgG4_Pro

Human anti Etanercept

Product Type
Monoclonal Antibody
Clone
AbD25940_hIgG4_Pro
Isotype
IgG4
Specificity
Etanercept

Product Code Applications Pack Size List Price Your Price Qty
HCA277
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human anti etanercept, clone AbD25940_hIgG4_Pro is an antibody that specifically recognizes etanercept as well as the extracellular ligand-binding portion of the 75 kDa (p75) human tumor necrosis factor receptor 2 (TNFR2). Clone AbD25940_hIgG4_Pro inhibits the binding of etanercept to its target tumour necrosis factor alpha (TNFα). The antibody binds to free etanercept but not to etanercept bound to its target TNFα. The antibody binds to an epitope which is also part of the TNFα binding site and can be used to measure the levels of etanercept and biosimilar products (e.g. Etacept, Intacept) in bioanalytical assays.

CloneAbD25940_hIgG4_Pro is a fully human recombinant monoclonal antibody with an IgG4 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD25939 (HCA276) in monovalent Fab format, as the capture antibody.

Etanercept (Enbrel®) is a dimeric fusion protein consisting of the extracellular ligand-binding portion of the human 75 kDa (p75) TNFR2 linked to the Fc portion of human IgG1. The Fc component of etanercept contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It is used to treat autoimmune diseases by interfering with TNF, acting as a TNF inhibitor. It binds to both TNFα and TNFβ (Lymphotoxin A).

View a summary of all anti-etanercept antibodies.

Product Form
Human IgG4 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Etanercept
Affinity
The intrinsic affinity of the monovalent form of this antibody is KD=2.4 nM as measured by real time, label-free molecular interaction analysis on immobilized Etanercept.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Enbrel is a trademark of Immunex Corporation.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used in a direct or indirect ELISA or as a detection antibody in a bridging ELISA. Protocol: ADA bridging ELISA.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Etanercept HCA276 E 0.1 mg loader
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Description Human anti Etanercept
Human anti Etanercept:HRP HCA277P E 0.1 mg loader
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Description Human anti Etanercept:HRP
Human anti Etanercept HCA279 E 0.1 mg loader
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Description Human anti Etanercept
Human anti Etanercept:HRP HCA279P E 0.1 mg
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Description Human anti Etanercept:HRP

Synonyms
ENBREL®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA277

151566

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with ETANERCEPT specificity

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