Elotuzumab antibody | AbD60180ia
Human anti Elotuzumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD60180ia
- Isotype
- IgG1
- Specificity
- Elotuzumab
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Elotuzumab Antibody, clone AbD60180ia (HCA438) at a concentration of 2 μg/mL in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with elotuzumab, human VH/VL subclass control, Rituximab, Alemtuzumab and Trastuzumab at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Elotuzumab Antibody, clone AbD60180ia (HCA438) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data for elotuzumab are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with elotuzumab at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Elotuzumab Antibody, clone AbD60184ia (HCA440, black), clone AbD60183ia (HCA439, red), or clone AbD60180ia (HCA438, blue). Detection was performed using biotin conjugated Elotuzumab at a concentration of 2 µg/mL in PBST followed by HRP conjugated Neutravidin in HISPEC Assay Diluent (BUF049A). Biotin conjugation of the antibody used in this assay was performed using a LYNX Rapid Plus Biotin (Type 1) Antibody Conjugation Kit (LNK263B). Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with Neutravidin at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the plate was coated with human SLAM7-His-bio at a concentration of 1 µg/mL and washed. A pre-incubated mixture of Elotuzumab (0.15 µg/mL) plus increasing concentrations of Human Anti-Elotuzumab Antibody, clone AbD60180ia (HCA438) was added. Free elotuzumab, still capable of binding to the human SLAM7 coated plate, was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-Elotuzumab Antibody, clone AbD60180ia is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug elotuzumab, and its biosimilar products. It does not recognize the drug target, signalling lymphocytic activation molecule family member 7 protein (SLAMF7), nor elotuzumab in complex with SLAMF7. This antibody can be used to measure free elotuzumab levels in serum from patients. HCA438 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 3.7nM and is one of the three antibodies offered for ADA assays. Antibody HCA439 has an affinity or 0.9nM and HCA440 has an affinity of 0.7nM. Elotuzumab (trade name Empliciti) is a humanized monoclonal antibody of the IgG1/kappa isotype that targets SLAMF7 and directly activates Natural Killer cells through both the SLAMF7 pathway and immunostimulatory Fc receptor binding. Elotuzumab also targets SLAMF7 on myeloma cells and facilitates the interaction with Natural Killer cells to mediate the killing of myeloma cells through antibody-dependent cellular cytotoxicity (ADCC). Elotuzumab is indicated to treat multiple myeloma and in combination with lenalidomide and dexamethasone for the treatment of patients with multiple myeloma. View a summary of all anti-elotuzumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This product is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Elotuzumab
- Affinity
- The monovalent intrinsic affinity of Human Anti-Elotuzumab Antibody, clone AbD60180ia was measured as KD = 3.7 nM by real time, label-free molecular interaction analysis on immobilized elotuzumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Empliciti is a registered trademark of Bristol-Myers Squibb and AbbVie Inc. - Licensed Use
- For in vitro. research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Clone AbD60180ia can be used in indirect ELISA to detect elotuzumab. It can also be used as a surrogate positive control or a calibrator in an anti-drug antibody (ADA) assay, together with HCA439 and HCA440.
Protocol: ADA bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Elotuzumab | HCA439 | E | 0.1 mg |
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| Description | Human anti Elotuzumab | ||||||
| Human anti Elotuzumab | HCA440 | E | 0.1 mg |
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| Description | Human anti Elotuzumab | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Human anti Elotuzumab | TZA0115 | E | 0.1 mg |
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| Description | Human anti Elotuzumab | ||||||
| Human anti Elotuzumab | TZA0116 | E | 0.1 mg |
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| Description | Human anti Elotuzumab | ||||||
| Human anti Elotuzumab:HRP | TZA0116P | E | 0.1 mg |
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| Description | Human anti Elotuzumab:HRP | ||||||
- Synonyms
- Empliciti
HCA438
171829
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Elotuzumab specificity
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