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Durvalumab antibody | AbD41240

Human anti Durvalumab (Drug/Target Complex)

Product Type
Monoclonal Antibody
Fab antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Durvalumab (Drug/Target Complex) Antibody, clone AbD41240 specifically recognizes the antibody drug durvalumab when in complex with its target, PD-L1 (programmed cell death ligand 1). The antibody does not recognize free durvalumab nor unbound recombinant human PD-L1. It can be used to measure levels of durvalumab in patient samples.

This TrailBlazer Antibody is available in two different formats, monovalent Fab unconjugated (TZA002) and bivalent Fab conjugated to HRP (TZA002P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free durvalumab captured via immobilized PD-L1.

The antibody is tagged with a SpyTag2 at the C-terminus of the Fab antibody heavy chain, enabling the user to couple it to any of the SpyCatchers in our catalog for conversion to alternative formats in less than one hour.

View a summary of all SpyCatcher products

Durvalumab (Imfinzi) is a human IgG1/kappa antibody with allotype G1m3. The CH2 domain was engineered with three amino acid substitutions (L238F, L239E, P335S) to reduce the Fc-mediated effector functions to prevent depletion of PD-L1+ T-cells. It was first approved for previously treated patients with advanced bladder cancer. It was later approved for the treatment of unresectable stage 3 NSCLC (Non-Small Cell Lung Cancer) and ES-SCLC. Durvalumab binds to PD-L1 to block the interaction with the inhibitory T cell receptor PD-1. This blockade removes the suppression of T-cells that are specific for MHC-1-presented neoepitopes on tumor cells. Removal of this inhibitory signal leads to the release of granzymes and perforins by the activated T-cells, and finally to the destruction of the tumor cells.

View a summary of all anti-durvalumab antibodies

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
PD-L1/Durvalumab complex
The monovalent intrinsic affinity of AbD41240ad was measured as KD = 13 nM by real time, label free molecular interaction analysis on immobilized PD-L1/dupilumab complex.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
His-tag is a registered trademark of EMD Biosciences.
Imfinzi is a trademark of Medimmune/AstraZeneca using Abgenix`s XenoMouse® technology.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Clone AbD41240ad can be used as the detection antibody in a PK Antigen Capture ELISA to measure durvalumab in complex with PD-L1.

Protocol: PK Antigen Capture ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
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Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Durvalumab HCA359 E 0.1 mg loader
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Description Human anti Durvalumab
Human anti Durvalumab HCA360 E 0.1 mg loader
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Description Human anti Durvalumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Human anti Durvalumab (Drug/Target Complex):HRP TZA002P E 0.1 mg
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Description Human anti Durvalumab (Drug/Target Complex):HRP
Human anti Durvalumab TZA004 E 0.1 mg
List Price Your Price
Description Human anti Durvalumab

Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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