IgY antibody | 16C7
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Mouse anti Duck IgY Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- 16C7
- Isotype
- IgG1
- Specificity
- IgY
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Mouse anti Duck IgY antibody, clone 16C7 recognises both the IgY and IgY(-Fc) Pekin duck heavy chain molecules. The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. The IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab′)2 fragment of IgY. Mouse anti Duck IgY antibody, clone 16C7 also detects IgY heavy chain in Mallard ducks, goose and swan. Mouse anti Duck IgY antibody, clone 16C7 detects bands of approximately 118 kDa and 178-200 kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting. |
- Target Species
- Duck
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chicken Goose - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified Pekin duck yolk IgY.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
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| Western Blotting | |
non-reducing |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | C E WB | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
Further Reading
-
Magor, K.E. et al. (1994) One gene encodes the heavy chains for three different forms of IgY in the duck.
J Immunol. 153 (12): 5549-55. -
Higgins, D.A. & Warr, G.W. (1993) Duck immunoglobulins: structure, functions and molecular genetics.
Avian Pathol. 22 (2): 211-36.
- RRID
- AB_609819
MCA2481
151695
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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