IgY antibody | 16C7

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Mouse anti Duck IgY Heavy Chain

Product Type
Monoclonal Antibody
Clone
16C7
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2481 E F WB 0.25 mg
Mouse anti Duck IgY antibody, clone 16C7 recognises both the IgY and IgY(-Fc) Pekin duck heavy chain molecules.

The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. The IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab′)2 fragment of IgY.

Mouse anti Duck IgY antibody, clone 16C7 also detects IgY heavy chain in Mallard ducks, goose and swan.

Mouse anti Duck IgY antibody, clone 16C7 detects bands of approximately 118 kDa and 178-200 kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.

Product Details

Target Species
Duck
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Purified Pekin duck yolk IgY.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgY antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Western Blotting non-reducing
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB

Product Specific References

Further Reading

  1. Magor, K.E. et al. (1994) One gene encodes the heavy chains for three different forms of IgY in the duck.
    J Immunol. 153 (12): 5549-55.
  2. Higgins, D.A. & Warr, G.W. (1993) Duck immunoglobulins: structure, functions and molecular genetics.
    Avian Pathol. 22 (2): 211-36.