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MHC Class II Monomorphic antibody | YKIX334.2

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Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 thumbnail image 1 Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 thumbnail image 2 Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 thumbnail image 3 Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 thumbnail image 4 Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 thumbnail image 5 Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 thumbnail image 6
Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 gallery image 1
Published customer image:
Rat anti Canine MHC Class II antibody, clone YKIX334.2 (MCA1044GA) used for immunolabelling of canine skin sections by immunohistochemistry on cryostat sections.
Image caption:
Immunostained sections. (A) MHC-II positivity in fresh tracheal epithelium (↑); (B) intraepithelial MHC-II after 9 months of cryopreservation, confirmed by immunostaining (↑) (immunohistochemical stain). MHC, major histocompatibility complex.

From: Lu T, Huang Y, Liu Y, Shen Y, Qiao Y, Zhang Y.
Effects of cryopreservation on tracheal allograft antigenicity in dogs.
J Thorac Dis. 2017 Jul;9(7):2038-47.
Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 gallery image 2
Staining of canine peripheral blood lymphocytes with FITC conjugated Rat anti Canine MHC Class II antibody, clone YKIX334.2 (MCA1044F)
Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 gallery image 3
Published customer image:Rat anti Canine MHC Class II antibody, clone YKIX334.2 (MCA1044GA) used for evaluation of MHC class II expression on canaine cells by flow cytometry.
Image caption:
CD11b+CD14−MHCII− cells suppress T cell proliferation. Facs sorted CD11b+CD14−MHCII− cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 gallery image 4
Published customer image:Rat anti Canine MHC Class II antibody, clone YKIX334.2 (MCA1044GA) used for evaluation of MHC class II expression on canine cells by flow cytometry.
Image caption:
CD11b+CD14+MHCII− cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII− cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean ± SEM are shown.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 gallery image 5
Published customer image:Rat anti Canine MHC Class II antibody, clone YKIX334.2 (MCA1044GA) used for evaluation of MHC class II expression on canaine cells by flow cytometry.
Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14−MHCII− cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14−MHCII− cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14−MHCII− cells is shown at 63× magnification.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
Anti Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 gallery image 6
Figure A. Alexa Fluor 647 conjugated Rat anti Dog CD5 (MCA1037A647) and FITC conjugated Rat IgG2a isotype control (MCA6005F). Figure B. Alexa Fluor 647 conjugated Rat anti Dog CD5 (MCA1037A647) and FITC conjugated Rat anti Dog MHC Class II (MCA1044F). All experiments performed on red cell lysed canine blood gated on live single lymphocytes, in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer.

Rat anti Dog MHC Class II Monomorphic:FITC

Rat anti Dog MHC Class II Monomorphic

Product Type
Monoclonal Antibody
Clone
YKIX334.2
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1044F
Datasheet
F
SDS
100 Tests loader

MCA1044GA
Datasheet
C F IP
SDS
0.1 mg loader

Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti Dog MHC Class II immunoprecipitates an antigen of ~32/34 kDa and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.

Product Details

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hooded Seal
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Con A activated canine peripheral blood cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
Rat IgG2a Negative Control:FITC MCA6005F F 0.1 mg loader

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for MHC Class II Monomorphic antibody

  1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
    Tissue Antigens. 43 (3): 137-54.
  2. Watson, C.J. et al. (1994) Immunosuppression of canine renal allograft recipients by CD4 and CD8 monoclonal antibodies.
    Tissue Antigens. 43 (3): 155-62.
  3. Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.
    Clin Exp Immunol.146: 303-11.
  4. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
    Vet Immunol Immunopathol. 141: 64-75.
  5. Bonnefont-Rebeix, C. et al. (2007) Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC).
    Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):134-9.
  6. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.
    Cell Immunol. 263: 88-98.
  7. Mito, K. et al. (2010) IFNγ markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models.
    Cancer Res. 70: 7093-101.
  8. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
    Am J Trop Med Hyg. 70: 618-24.
  9. Schütze, N. et al. (2009) Inactivated parapoxvirus ovis activates canine blood phagocytes and T lymphocytes.
    Vet Microbiol. 137: 260-7.
  10. Liu, Y. et al. (2000) Immunosuppressant-free allotransplantation of the tracheaThe antigenicity of tracheal grafts can be reduced by removing the epithelium and mixed glands from the graft by detergent treatment.
    J Thorac Cardiovasc Surg. 120: 108-14.
  11. Larsen, A.K. et al. (2013) Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro.
    PLoS One. 8: e70186.
  12. Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
    Immunobiology. 221 (1): 12-22.
  13. Lin, S-C. et al. (2014) Immune Characterization of Peripheral Blood Mononuclear cells of the Dogs Restored from Innoculation of Canine Transmissible Venereal Tumor Cells.
    Tai Vet J. 40 (04): 181-90.
  14. Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
    Parasite Immunol. Jul 27 [Epub ahead of print].
  15. Lu, T. et al. (2017) Effects of cryopreservation on tracheal allograft antigenicity in dogs.
    J Thorac Dis. 9 (7): 2038-2047.

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