CD45 antibody | YKIX716.13
Mouse anti Canine CD45 antibody, clone YKIX716.13 (MCA1042GA) used as part of a panel of antibodies to demonstrate the phenotype of canine mesenchymal stem cells by flow cytometry together with mesenchymal markers CD9, CD44, CD90 and CD105. Negative results for lineage cell markers CD34, CD45 and STRO-1 are shown.
Image caption:
b. Representative histograms demonstrating positive and negative staining of marrow cMSCs from a single donor
From: Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB.
In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 2017 Oct 3;8(1):218.
Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 (FL2) and CD61 (FL1) antibody-labeled samples. Average CD61+: 99.47 ± 0.21% (n=3). Average CD45+: 0.19 ± 0.04% (n=3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).
From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C.
Ultra-pure platelet isolation from canine whole blood.
BMC Vet Res. 2013 Jul 17;9:144.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Canine CD45 antibody, clone YKIX716.13 (MCA1042GA) used for the evaluation of CD45 expression on canine bone marrow-derived mesenchymal stem cells by flow cytometry.
Image caption:
The phenotype of cultured bone marrow-derived mesenchymal stem cells (BM-MSCs) from each dog was confirmed before autologous injection.
Expression of CD29, CD34, CD44, CD45, and CD90 by cultured cells was evaluated by flow cytometric analysis using anti-CD29, anti-CD34, anti-CD44, anti-CD45, and anti-CD90 antibodies, after selection of the cell population of interest. Control isotype antibody is plotted in red in each histogram plot. Cultured cells were confirmed as BM-MSCs by their appearance in culture and by the CD29+CD44+CD90+CD34-CD45- phenotype.
From: Muir P, Hans EC, Racette M, Volstad N, Sample SJ, Heaton C, et al. (2016)
Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
PLoS ONE 11(8): e0159095.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F ResetRat anti Dog CD45
- Product Type
- Monoclonal Antibody
- Clone
- YKIX716.13
- Isotype
- IgG2b
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Rat anti Dog CD45 antibody, clone YKIX716.13 recognizes canine CD45 also known as leukocyte common antigen lustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop (CLAW). Clone YKIX 716.13: immunoprecipitates an antigen of ~180/200 kDa from Con-A blasts (Cobbold et al. 1994). CD45 is expressed on all leukocytes in canine peripheral blood. Rat anti Dog CD45 antibody, clone YKIX716.13 reacts with CD45 on all outbred mongrels and beagles tested and may be against CD45RB isoform. |
- Target Species
- Dog
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Canine thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2b Negative Control | ||||||
References for CD45 antibody
-
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54. -
Reis, A.B. et al (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.
Clin Exp Immunol.146: 303-11. -
Stein, V.M. et al. (2008) Immunophenotypical characterization of monocytes in canine distemper virus infection.
Vet Microbiol. 131:237-46. -
Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
Am J Trop Med Hyg. 70: 618-24. -
Tominaga, M. et al. (2010) Flow cytometric analysis of peripheral blood and tumor-infiltrating regulatory T cells in dogs with oral malignant melanoma.
J Vet Diagn Invest. 22: 438-41. -
Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.
J Nutr. 132: 1652S-4S. -
Hunter, M.J. et al. (2011) Gene therapy of canine leukocyte adhesion deficiency using lentiviral vectors with human CD11b and CD18 promoters driving canine CD18 expression.
Mol Ther. 19: 113-21. -
Comazzi, S. et al. (2006) Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45.
Vet Clin Pathol. 35: 47-54.
View The Latest Product References
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Giantin, M. et al. (2013) Evaluation of tyrosine-kinase receptor c-KIT (c-KIT) mutations, mRNA and protein expression in canine leukemia: might c-KIT represent a therapeutic target?
Vet Immunol Immunopathol. 152: 325-32. -
Trichler, S.A. et al. (2013) Ultra-pure platelet isolation from canine whole blood.
BMC Vet Res. 9: 144. -
Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.
Vet Comp Oncol. 12: 29-36. -
Salinas, L.T. et al. (2015) Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system.
Brain Behav Immun. 50: 155-65. -
Muir, P. et al. (2016) Autologous Bone Marrow-Derived Mesenchymal Stem Cells Modulate Molecular Markers of Inflammation in Dogs with Cruciate Ligament Rupture.
PLoS One. 11 (8): e0159095. -
Poggi, A. et al. (2017) Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high-grade B-cell lymphoma.
Vet Comp Oncol. 15 (2): 431-40. -
Zeira, O. et al. (2015) Adult autologous mesenchymal stem cells for the treatment of suspected non-infectious inflammatory diseases of the canine central nervous system: safety, feasibility and preliminary clinical findings.
J Neuroinflammation. 12: 181. -
Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
Vet Immunol Immunopathol. 159 (1-2): 91-6. -
Michael, H.T. et al. (2013) Isolation and characterization of canine natural killer cells.
Vet Immunol Immunopathol. 155 (3): 211-7. -
Nishimura, T. et al. (2017) Feeder-independent canine induced pluripotent stem cells maintained under serum-free conditions.
Mol Reprod Dev. 84 (4): 329-39. -
Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
Immunobiology. 221 (1): 12-22. -
Bearden, R.N. et al. (2017) In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.
Stem Cell Res Ther. 8 (1): 218. -
Lee, S.H. et al. (2016) Impact of local injection of brain-derived neurotrophic factor-expressing mesenchymal stromal cells (MSCs) combined with intravenous MSC delivery in a canine model of chronic spinal cord injury.
Cytotherapy. S1465-3249(16)30540-0. -
Hansmann, F. et al. (2018) Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
Vet Immunol Immunopathol. 202: 130-40. -
Aricò, A. et al. (2013) The role of vascular endothelial growth factor and matrix metalloproteinases in canine lymphoma: in vivo and in vitro study.
BMC Vet Res. 9: 94. -
Reineking, W. et al. (2018) Canine primary jejunal and colonic epithelial cells predominantly express TLR5 and TLR9 but do not change TLR expression pattern after stimulation with certain Toll-like receptor ligands.
Vet Immunol Immunopathol. 206: 16-24. -
Martini, V. et al. (2019) Prognostic role of non-neoplastic lymphocytes in lymph node aspirates from dogs with diffuse large B-cell lymphoma treated with chemo-immunotherapy.
Res Vet Sci. 125: 130-5. -
Wolf-Ringwall, A. et al. (2020) Prospective evaluation of flow cytometric characteristics, histopathologic diagnosis and clinical outcome in dogs with naïve B-cell lymphoma treated with a 19-week CHOP protocol.
Vet Comp Oncol. 18 (3): 342-52. -
Sayag, D. et al. (2020) Proof-of-concept study: Evaluation of plasma and urinary electrolytes as markers of response to L-asparaginase therapy in dogs with high-grade lymphoma.
Vet Clin Pathol. 49 (3): 476-83. -
Lee, J. et al. (2021) Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor.
Biomed Res Int. 2021: 6690704. -
Wi, H. et al. (2021) Immunosuppression-enhancing effect of the administration of allogeneic canine adipose-derived mesenchymal stem cells (cA-MSCs) compared with autologous cA-MSCs in vitro.
J Vet Sci. 22 (5): e63. -
Grudzien, M. et al. (2021) A newly established canine NK-type cell line and its cytotoxic properties.
Vet Comp Oncol. 19 (3): 567-577. -
Stein, L. et al. (2021) Immunophenotypic Characterization of Canine Nodal T-Zone Lymphoma.
Vet Pathol. 58 (2): 288-92. -
Grandi, F. et al. (2022) Immunophenotypic and molecular profile of cancer stem-cell markers in ex vivo canine transmissible venereal tumour (CTVT).
Vet Med Sci. 8 (6): 2297-306.
MCA1042GA
160278
163381
1711
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