Denosumab antibody | AbD26296

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Human anti Denosumab

Anti-denosumab antibody is a recombinant, inhibitory anti-idiotypic antibody in monovalent Fab format; it is recommended as capture antibody in a PK bridging ELISA format with HCA280 for detection.

Product Type
Monoclonal Antibody
Clone
AbD26296
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA288 E 0.1 mg
Human Anti-Denosumab Antibody, clone AbD26296 is a paratope specific, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug denosumab. The antibody does not recognize free RANKL (receptor activator of nuclear factor kappa-B ligand), or denosumab in complex with human RANKL and can be used to measure the levels of free denosumab and biosimilar products in bioanalytical assays.

A pair of anti-denosumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with an anti-denosumab antibody in full immunoglobulin format, clone AbD26295_hIgG1 (HCA280) as the detection antibody.

Denosumab (Prolia, Xgeva) is a fully human monoclonal antibody (IgG2/kappa) for the treatment of osteoporosis, treatment-induced bone loss, bone metastases, multiple myeloma, and giant cell tumor of bone. The drug specifically binds to human RANKL, a protein that acts as the primary signal to promote bone removal/resorption and prevents RANKL from activating its receptor, RANK, on the surface of osteoclasts and their precursors.

View a summary of all anti-denosumab antibodies.

Product Details

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Denosumab
Affinity
The monovalent intrinsic affinity of AbD26296 was measured as KD = 7.1 nM by real time, label free molecular interaction analysis on immobilized denosumab
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Prolia and Xgeva are registered trademarks of Amgen Inc.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Denosumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD26296 can be used in a direct or indirect ELISA system or as capture antibody for denosumab in a bridging ELISA together with HCA280P (AbD26295_hIgG1) as the detection reagent.
Protocol: PK bridging ELISA to measure total drug

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Denosumab HCA280 0.1 mg E
Human anti Denosumab:HRP HCA280P 0.1 mg E
Human anti Denosumab HCA281 0.1 mg E
Human anti Denosumab HCA282 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK001P 1 Conjugation For 400µg Antibody CJ
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB