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Daratumumab antibody | AbD45015ia

Human anti Daratumumab

Product Type
Monoclonal Antibody

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Daratumumab Antibody, clone AbD45015ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody daratumumab. It does not recognize free CD38 nor daratumumab in complex with CD38. The antibody can be used to measure free daratumumab in patient samples.

This anti-daratumumab antibody can be used as both capture and detection reagent to develop a pharmacokinetic (PK) bridging assay to measure free drug.

Clone AbD45015ia is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 0.9 nM, and is one of three antibodies with different affinities offered for ADA assays.

Daratumumab (Darzalex) is a human IgG1/kappa antibody generated using transgenic mouse technology. It has been approved for the treatment of patients with multiple myeloma. It has orphan drug designation for multiple myeloma, diffuse large B cell lymphoma, follicular lymphoma, and mantle cell lymphoma. The antibody drug targets CD38, overexpressed by multiple myeloma cells, and indirectly induces apoptosis. In addition, daratumumab modulates CD38 enzymatic activity, inhibiting the cyclase enzyme activity and stimulating the hydrolase activity (Van de Donk et al. 2016).

View a summary of all anti-daratumumab antibodies
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Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
The intrinsic affinity of the monovalent form of Human anti Daratumumab antibody, clone AbD45015ia is KD = 0.9 nM as measured by real time, label free molecular interaction analysis on immobilized Daratumumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
Darzalex is a trademark of Janssen Biotech, Inc.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Human anti Daratumumab antibody, clone AbD45015ia can be used as capture and detection antibody for Daratumumab in a bridging ELISA to measure free drug. It can also be used as a calibrator in an anti-drug antibody assay.

Protocol: ADA bridging ELISA.
Protocol: PK bridging ELISA.

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Further Reading

  1. van, N.D.D. et al. (2016) Monoclonal antibodies targeting CD38 in hematological malignancies and beyond.
    Immunol Rev. 270 (1): 95-112.



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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