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Daratumumab antibody | AbD44786ia

Human anti Daratumumab

Product Type
Monoclonal Antibody
Clone
AbD44786ia
Isotype
IgG1
Specificity
Daratumumab

Product Code Applications Pack Size List Price Your Price Qty
HCA366
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Daratumumab Antibody, clone AbD44786ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody daratumumab. It does not recognize free CD38 nor daratumumab in complex with CD38. The antibody can be used to measure free daratumumab in patient samples.

Clone AbD44786ia is a fully human recombinant monoclonal antibody with IgG1 isotype and can be used as a reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 9 nM, and is one of three antibodies with different affinities offered for ADA assays.

Daratumumab (Darzalex) is a human IgG1/lambda antibody generated using transgenic mouse technology. It has been approved for the treatment of patients with multiple myeloma. It has orphan drug designation for multiple myeloma, diffuse large B cell lymphoma, follicular lymphoma, and mantle cell lymphoma. The antibody drug targets CD38, overexpressed by multiple myeloma cells, and indirectly induces apoptosis. In addition, daratumumab modulates CD38 enzymatic activity, inhibiting the cyclase enzyme activity and stimulating the hydrolase activity (Van de Donk et al. 2016).

View a summary of all anti-daratumumab antibodies

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Daratumumab
Affinity
The intrinsic affinity of the monovalent form of Human anti Daratumumab antibody, clone AbD44786ia is KD = 9 nM as measured by real time, label free molecular interaction analysis on immobilized Daratumumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Darzalex is a trademark of Janssen Biotech, Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
Human anti Daratumumab antibody, clone AbD44786ia can be used as a calibrator in an anti-drug antibody assay.

Protocol: ADA bridging ELISA.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Daratumumab HCA364 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Daratumumab
Human anti Daratumumab HCA365 E 0.1 mg loader
List Price Your Price
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Description Human anti Daratumumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit

Further Reading

  1. van, N.D.D. et al. (2016) Monoclonal antibodies targeting CD38 in hematological malignancies and beyond.
    Immunol Rev. 270 (1): 95-112.

Synonyms
Darzalex
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA366

159797 166061

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