MHC Class II DQ antibody | CC158

Mouse anti Bovine MHC Class II DQ

Product Type
Monoclonal Antibody
Clone
CC158
Isotype
IgG2a
Specificity
MHC Class II DQ

Product Code Applications Pack Size List Price Your Price Qty
MCA5655
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Bovine MHC class II DQ antibody, clone CC158 recognises Bovine MHC Class II DQ. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. .

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References for MHC Class II DQ antibody

  1. Stephens, S.A. & Howard, C.J. (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
    Parasitology. 124 (Pt 5): 485-93.
  2. Yamakawa, Y. et al. (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN.
    J Leukoc Biol. 83 (6): 1396-403.
  3. Price,S.J. and Hope, J.C. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.
    Immunology. 126: 201-8.
  4. Van Rhijn, I. et al. (2007) Massive, sustained gammadelta T cell migration from the bovine skin in vivo.
    J Leukoc Biol. 81: 968-73.

Flow Cytometry

RRID
AB_10863323
UniProt
Q30308
View more products with MHC CLASS II specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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