IL-2 antibody | AbD21766
Recombinant bovine interleukin-2 detected using Human anti Bovine interleukin-2, clone AbD21766 (HCA267) as the capture antibody and Human anti Bovine interleukin-2 clone AbD22297 (HCA268) followed by Mouse anti Histidine Tag:HRP (MCA1396P) as the detection reagent.
PBMC from naturally Mycobacterium bovis infected cattle were stained using Human anti Bovine interleukin-2 antibody, clone AbD21766, conjugated with Alexa Fluor 647 (HCA267A467) at a dilution of 1:8.
Human anti Bovine Interleukin-2, clone AbD21766 recognizes bovine interleukin-2 (IL-2), also known as T-cell growth factor (TCGF). Bovine IL-2 is a 155 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.
Human anti bovine interleukin-2, clone AbD21766, has been successfully used for the detection and measurement of native and recombinant bovine IL-2 using intracellular flow cytometry, ELISPOT and ELISA.
In ELISA and ELISPOT clone AbD21766 functions as the capture antibody in a sensitive assay for bovine IL-2 determination.
Human anti bovine interleukin-2, clone AbD21766, has been successfully used for the detection and measurement of native and recombinant bovine IL-2 using intracellular flow cytometry, ELISPOT and ELISA.
In ELISA and ELISPOT clone AbD21766 functions as the capture antibody in a sensitive assay for bovine IL-2 determination.
Product Details
- Target Species
- Bovine
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to Alexa Fluor 647 (A647) - liquid.
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Fc-fusion protein containing the amino acid sequence 21-155 from bovine interleukin-2.
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.3 nM by real time, label-free molecular interaction analysis on recombinant bovine interleukin-2.
- Approx. Protein Concentrations
- Antibody concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
- Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P05016 Related reagents
- Entrez Gene
- IL2 Related reagents
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com - Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad
- Regulatory
- For research purposes only
Applications of IL-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| ELISA | |||
| ELISpot | |||
| Flow Cytometry |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with HCA268P as the detection reagent.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| HuCAL Fab-FH Negative Control | HCA045 | 0.1 mg | C E P WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Leucoperm™ | BUF09 | 50 Tests | F | ||
| 5x ELISA Coating Buffer | BUF030A | 100 ml | E | ||
| Human anti Bovine Interleukin-2 | HCA268 | 0.1 mg | E ES |
Product Specific References
Further Reading
-
Rhodes, S.G. et al. (2014) Use of antigen-specific interleukin-2 to differentiate between cattle vaccinated with Mycobacterium bovis BCG and cattle infected with M. bovis.
Clin Vaccine Immunol. 21:39-45. -
Whelan, A.O., et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected
with Bovine Tuberculosis
PLoS One. 6(12):e29194
