IL-2 antibody | AbD21766

Human anti Bovine Interleukin-2

Product Type
Monoclonal Antibody
Clone
AbD21766
Isotype
HuCAL Fab monovalent
Specificity
IL-2

Product Code Applications Pack Size List Price Your Price Qty
HCA267
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E ES F * 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Human anti Bovine Interleukin-2, clone AbD21766 recognizes bovine interleukin-2 (IL-2), also known as T-cell growth factor (TCGF). Bovine IL-2 is a 155 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.

Human anti bovine interleukin-2, clone AbD21766, has been successfully used for the detection and measurement of native and recombinant bovine IL-2 using intracellular flow cytometry, ELISPOT and ELISA.

In ELISA and ELISPOT clone AbD21766 functions as the capture antibody in a sensitive assay for bovine IL-2 determination.

Target Species
Bovine
Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Fc-fusion protein containing the amino acid sequence 21-155 from bovine interleukin-2.
Affinity
The intrinsic affinity of the monovalent form of this antibody is KD=0.3 nM as measured by real time, label-free molecular interaction analysis on recombinant bovine interleukin-2.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISpot
Flow Cytometry 1
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with HCA268P as the detection reagent.

Description Product Code Applications Pack Size List Price Your Price Quantity
HuCAL Fab-FH Negative Control HCA045 E 0.1 mg loader
List Price Your Price
loader
Description HuCAL Fab-FH Negative Control

Description Product Code Applications Pack Size List Price Your Price Quantity
5x ELISA Coating Buffer BUF030A E 100 ml
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Description 5x ELISA Coating Buffer
Human anti Bovine Interleukin-2 HCA268 E ES 0.1 mg loader
List Price Your Price
loader
Description Human anti Bovine Interleukin-2

Further Reading

  1. Rhodes, S.G. et al. (2014) Use of antigen-specific interleukin-2 to differentiate between cattle vaccinated with Mycobacterium bovis BCG and cattle infected with M. bovis.
    Clin Vaccine Immunol. 21:39-45.
  2. Whelan, A.O., et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis
    PLoS One. 6(12):e29194

UniProt
P05016
Entrez Gene
IL2
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad
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