CD8a antibody | 7C2B
A. Mouse IgG2a negative control (MCA929) followed by Goat anti Mouse IgG2a:PE (STAR133PE).
B. Mouse anti Bovine CD8a antibody, clone 7C2B (MCA6083) followed by Goat anti Mouse IgG2a:PE secondary antibody (STAR133PE).
Both the Mouse IgG2a negative control and Mouse anti Bovine CD8a are plotted against bovine CD40 as recognized by FITC conjugated Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431F).
Mouse anti Bovine CD8a, clone 7C2B, recognizes bovine CD8a. CD8a is a coreceptor for MHC I molecules. It is involved in the activation of the cytotoxic T-cell response and the clearance of virally infected cells. CD8 is predominantly present either as homodimer or a multimer of the alpha chain or as heterodimer of the alpha and beta chains (Littman et al. 1987). Bovine CD8 differs from the CD8 molecules of other animal species and shares 57.85% identity with swine, 37.70% with mouse, 27.50 % with chicken, and 20.69% identity with carp (Zhenbao et al. 2014).
Mouse anti Bovine CD8a, clone 7C2B recognizes bovine CD8 lymphocytes and gamma delta CD8+ lymphocytes (Park et al. 2000). In addition it reacts with the CD8 lymphocytes of water buffalo (Davis et al. 2001).
Mouse anti Bovine CD8a, clone 7C2B recognizes bovine CD8 lymphocytes and gamma delta CD8+ lymphocytes (Park et al. 2000). In addition it reacts with the CD8 lymphocytes of water buffalo (Davis et al. 2001).
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Sheep Bison Water Buffalo - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Concentrated tissue culture supernatant - liquid
- Preparation
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P31783
- Entrez Gene
- CD8A
- GO Terms
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of CD8a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Functional Assays |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG2a:RPE | STAR133PE | F | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Bovine CD3 | MCA6080 | F IC | 0.1 mg |
|
|
| Mouse anti Bovine CD4 | MCA6081 | F | 0.1 mg |
|
Product Specific References
References for CD8a antibody
-
Koo H.C. et al. (2004) Analysis of the immune response to Mycobacterium avium subsp. paratuberculosis in experimentally infected calves.
Infect Immun. 72 (12): 6870-83. -
Allen A.J. et al. (2009) Development of a bovine ileal cannulation model to study the immune response and mechanisms of pathogenesis of paratuberculosis.
Clin Vaccine Immunol. 16 (4): 453-63.
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