Mouse anti Bovine CD8a, clone 7C2B
, recognizes bovine CD8a. CD8a is a coreceptor for MHC I molecules. It is involved in the activation of the cytotoxic T-cell response and the clearance of virally infected cells. CD8 is predominantly present either as homodimer or a multimer of the alpha chain or as heterodimer of the alpha and beta chains (Littman et al. 1987
). Bovine CD8 differs from the CD8 molecules of other animal species and shares 57.85% identity with swine, 37.70% with mouse, 27.50 % with chicken, and 20.69% identity with carp (Zhenbao et al. 2014
Mouse anti Bovine CD8a, clone 7C2B recognizes bovine CD8 lymphocytes and gamma delta CD8+ lymphocytes (Park et al. 2000
). In addition it reacts with the CD8 lymphocytes of water buffalo (Davis et al. 2001
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Concentrated tissue culture supernatant - liquid
- Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
- Buffer Solution
- Serum free tissue culture medium containing proprietary protein free supplement
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD8a antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for CD8a antibody
Koo H.C. et al. (2004) Analysis of the immune response to Mycobacterium avium subsp. paratuberculosis in experimentally infected calves.
Infect Immun. 72 (12): 6870-83.
Allen A.J. et al. (2009) Development of a bovine ileal cannulation model to study the immune response and mechanisms of pathogenesis of paratuberculosis.
Clin Vaccine Immunol. 16 (4): 453-63.
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