CD4 antibody | CC8
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|Mouse anti Bovine CD4 antibody, clone CC8 recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Mouse anti Bovine CD4 antibody, clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskra et al. 1991).
A mutation in the bovine CD4 gene resulting in an amino acid substitution at A324 T, located in the D4 domain of the CD4 gene product can occur. This mutation results in lowered binding of Mouse anti Bovine CD4 antibody, clone CC8 to CD4 in Japanese Black (JB) cattle where this mutation has been identified (Kato-Mori, et al.. 2020). CD4 in JB cattle can be identified using clone CACT138A (MCA6081) whose binding to bovine CD4 is unaffected by the A324T mutation (Kato-Mori, et al.. 2020).
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin
- Bovine lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from an immunized mouse were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD4 antibody
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Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets.
Vet Immunol Immunopathol. 27 (1-3): 215-22.
Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
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Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34.
Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.
Infect Immun. 76: 3932-9.
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44.
Buddle, B.M. et al. (2003) Revaccination of neonatal calves with Mycobacterium bovis BCG reduces the level of protection against bovine tuberculosis induced by a single vaccination.
Infect Immun. 71: 6411-9.
View The Latest Product References
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Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells
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Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194.
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Dacal,. V. et al. (2013) Immunohistochemical characterization of inflammatory cells in the skin of cattle undergoing repeated infestations with Hypoderma lineatum (Diptera: Oestridae) larvae.
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Gondaira, S. et al. (2020) Immunosuppression in Cows following Intramammary Infusion of Mycoplasma bovis.
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