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CD32 antibody | CCG36

Mouse anti Bovine CD32:RPE

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price

Mouse anti Bovine CD32 antibody, clone CCG36 recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.

CD32 can function in an inhibitory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Bovine FcγRII-transfected COS7 cells.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS-1myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
For research purposes only
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

References for CD32 antibody

  1. Werling, D. et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus.
    Vet Immunol Immunopathol. 62 (3): 185-95.
  2. Chattha, K.S. et al. (2009) Age related variation in expression of CD21 and CD32 on bovine lymphocytes: a cross-sectional study.
    Vet Immunol Immunopathol. 130 (1-2): 70-8.
  3. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves.
    Vet Immunol Immunopathol. 137 (3-4): 284-90.
  4. Chattha, K.S. et al. (2010) Expression of complement receptor 2 (CD21), membrane IgM and the inhibitory receptor CD32 (FcgammaRIIb) in the lymphoid tissues of neonatal calves.
    Vet Immunol Immunopathol. 137 (1-2): 99-108.
  5. Chattha, K.S. et al. (2010) Variation in expression of membrane IgM, CD21 (CR2) and CD32 (Fcgamma RIIB) on bovine lymphocytes with age: a longitudinal study.
    Dev Comp Immunol. 34 (5): 510-7.

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0019864 IgG binding


165719 INN0512R

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