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CD11c antibody | BAQ153A

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Anti Bovine CD11c Antibody, clone BAQ153A thumbnail image 1 Anti Bovine CD11c Antibody, clone BAQ153A thumbnail image 2
Anti Bovine CD11c Antibody, clone BAQ153A gallery image 1
Flow cytometric staining of bovine peripheral blood monocytes.
A. Mouse IgM negative control (MCA692) followed by Goat anti Mouse IgM:PE (STAR86PE).
B. Mouse anti Bovine CD11c antibody, clone BAQ153A (MCA6084) followed by Goat anti Mouse IgM:PE secondary antibody (STAR86PE).
Both the Mouse IgM negative control and Mouse anti Bovine CD11c are plotted against bovine CD11b as recognized by FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F).
Anti Bovine CD11c Antibody, clone BAQ153A gallery image 2
Published customer image:
Mouse anti Bovine CD11c, clone BAQ153A (MCA6084) used for the analysis of CD11c expression on ovine and bovine cells by flow cytometry.
Image caption:
Identification of neonatal intestinal mononuclear phagocytes in the ileal Peyer’s patch of lambs and calves. Intestinal tissues from the ileal Peyer’s patch (IPP) were recovered from 10-day-old lambs (n = 6) and calves (n = 10). Mechanical and enzymatic dissociations were performed to obtain total isolated intestinal cells, then stained for flow cytometry. Representative gating for mononuclear phagocytes (MP) subsets are shown for the IPP of lamb (A) and calf (B). Following exclusion of dead cells, CD11c+MHCII+ cells were gated to select total MP and then four subsets were distinguished based on surface expression of CD14, CD172α, CD11b, and Cadm1: CD14+CD172α+Cadm1intCD11b+ (subset 1, green), CD14−CD172α−Cadm1+CD11b− (subset 2, purple), CD14−CD172α+Cadm1intCD11b+ (subset 3, blue), and CD14−CD172α+/-Cadm1−CD11b− (subset 4, yellow). Subsets 1, 2, and 3 of MP from the IPP of a 10-day-old lamb were cell sorted by flow cytometry, cytospined, and stained with hematoxylin and eosin. Pictures of the three subsets of MP were obtained by optical microscopy (A, right panel). Pie charts represent the cell proportions in percentage of each intestinal MP subset among viable CD11c+MHCII+ cells (mean ± SD) for lambs (n = 6; A, bottom panel) and calves (n = 10; B, bottom panel).

From: Baillou A, Tomal F, Chaumeil T, Barc C, Levern Y, Sausset A, Pezier T, Schulthess J, Peltier-Pain P, Laurent F and Lacroix-Lamandé S
Characterization of intestinal mononuclear phagocyte subsets in young ruminants at homeostasis and during Cryptosporidium parvum infection.
Front. Immunol. (2024) 15:1379798.
doi: 10.3389/fimmu.2024.1379798
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Bovine CD11c

Product Type
Monoclonal Antibody
Clone
BAQ153A
Isotype
IgM
Specificity
CD11c

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Product Code Applications Pack Size List Price Your Price Qty
MCA6084
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IC 0.1 mg loader
List Price Your Price
loader

Mouse anti Bovine CD11c, clone BAQ153A, recognizes bovine CD11c also known as integrin alpha X. CD11c is a receptor for fibrinogen recognizing the G-P-R motif in fibrinogen. It is involved in the cell to cell interaction during inflammatory responses. In addition, it has an important function in adhesion and chemotaxis in monocytes, macrophages and dendritic cells (DCs).

In cattle, CD11c+ DCs are a source of the anti-inflammatory cytokine IL-10 during infection by the highly pathogenic foot-and-mouth disease virus (FMDV). IL-10 may be responsible for the reduction in the antigen processing ability of DCs during FMDV infection (Sei et al. 2016). Clone BAQ153A reacts with bovine CD11c+ macrophages, DCs and some lymphocytes as shown by IHC (Palmer et al. 2011) and flow cytometry (Sei et al. 2016).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
Bison
Water Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated tissue culture supernatant - liquid
Preparation
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Cells from multiple species
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Functional Assays
Immunocytochemistry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Bovine CD14 MCA6085 F 0.1 mg
List Price Your Price
Description Mouse anti Bovine CD14

References for CD11c antibody

  1. Srsen S. et al. (1989) Jacob's syndrome (2 case reports).
    Cesk Pediatr. 44 (7): 420-2.
  2. Naessens, J. & Hopkins, J. (1996) Introduction and summary of workshop findings
    Veterinary Immunology and Immunopathology. 52 (4): 213-35.
  3. Jeyaseelan S. et al. (2001) Mannheimia haemolytica leukotoxin activates a nonreceptor tyrosine kinase signaling cascade in bovine leukocytes, which induces biological effects.
    Infect Immun. 69 (10): 6131-9.
  4. Elnaggar, M.M. et al. (2016) Characterization and use of new monoclonal antibodies to CD11c, CD14, and CD163 to analyze the phenotypic complexity of ruminant monocyte subsets.
    Vet Immunol Immunopathol. 178: 57-63.
  5. Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
    Ticks Tick Borne Dis. 11 (3): 101365.
  6. Baillou, A. et al. (2024) Characterization of intestinal mononuclear phagocyte subsets in young ruminants at homeostasis and during Cryptosporidium parvum infection
    Frontiers in Immunology. 15: 1379798 [Epub ahead of print].

MCA6084

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