CSF1R antibody | ROS-AV170
Mouse anti Chicken CSF1R:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- ROS-AV170
- Isotype
- IgG1
- Specificity
- CSF1R
Mouse anti Chicken CSF1R, clone ROS-AV170, recognizes the chicken homologue of human CSF1R, also known as CD115, CSF-1R, M-CSF receptor and c-fms. CSF1R is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010). CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cells (Garceau et al. 2010).Mouse anti Chicken CSF1R, clone ROS-AV170 is a monoclonal antibody that has been generated to recognize chicken CSF1R . Clone ROS-AV170 has been shown to recognize the CSF1R antigen in some, but not all closely related avian species. Mouse anti Chicken CSF1R, clone ROS-AV170 is an excellent marker for chicken macrophages. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goose Grey partridge Guinea Fowl Duck Quail Turkey Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Purified ChCSF1R-Fc fusion protein
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Immunofluorescence |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Mouse anti Chicken CD45:RPE | MCA2413PE | F | 100 Tests | Log in | |||
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Description | Mouse anti Chicken CD45:RPE | ||||||
Mouse anti Chicken Monocytes/Macrophages | MCA5770 | C F P * | 0.25 mg | Log in | |||
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Description | Mouse anti Chicken Monocytes/Macrophages | ||||||
Mouse anti Chicken CD25 | MCA5925GA | E F WB | 0.1 mg | Log in | |||
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Description | Mouse anti Chicken CD25 | ||||||
Mouse anti Chicken CD14 | MCA5926GA | E WB | 0.1 mg | Log in | |||
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Description | Mouse anti Chicken CD14 |
Source Reference
-
Garcia-Morales, C. et al. (2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.
Dev Comp Immunol 42:278-85.
References for CSF1R antibody
-
Garceau, V. et al. (2015) The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor.
BMC Biol. 13:12. -
Nagy, N. et al. (2016) Avian dendritic cells: Phenotype and ontogeny in lymphoid organs.
Dev Comp Immunol. 58: 47-59. -
Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Frontiers in Veterinary Science. 23 May [Epub ahead of print].
Further Reading
-
Garceau, V. et al. (2010) Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products.
J Leukoc Biol. 87:753-64.
- Synonyms
- CD115
MCA5956A488
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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