CD8 Beta antibody | EP42

100% Secure

Mouse anti Chicken CD8 Beta

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.25 mg

Mouse anti Chicken CD8 beta antibody, clone EP42 recognises Chicken CD8β. CD8 is a dimer formed of either two alpha chains, or an alpha chain and a beta chain.Mouse anti Chicken CD8 beta antibody, clone EP42 is specific for Chicken CD8β, binding to an epitope expressed on the CD8β chain, available only on cells expressing alpha/beta heterodimers.

Product Details

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Borate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

For research purposes only

Applications of CD8 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for CD8 Beta antibody

  1. Tregaskes, C.A .et al. (1995) Identification and analysis of the expression of CD8 alpha beta and CD8 alpha alpha isoforms in chickens reveals a major TCR-gamma delta CD8 alpha beta subset of intestinal intraepithelial lymphocytes.
    J Immunol. 154 (9): 4485-94.
  2. Imhof, B.A. et al. (2000) Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires.
    J Immunol. 165: 6716-22.
  3. Katevuo, K. and Vainio, O. (1996) Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery.
    Immunology. 89: 419-23.
  4. Luhtala, M. et al. (1995) Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes.
    Scand J Immunol. 42 (1): 171-4.
  5. Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8 alpha molecule.
    Poult Sci. 78 (11): 1526-31.
  6. Paramithiotis E et al. (1991) High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes.
    J Immunol. 147 (11): 3710-7.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra