CD8 Beta antibody | EP42
Mouse anti Chicken CD8 beta antibody, clone EP42 (MCA5763 used to label CD8b cells in chickin conjunctiva associated lymphoid tissue by immunofluorescence.
Image caption:
Cellular infiltrates of head-associated lymphoid tissues following vaccination with BeauR-M41(S1) and BeauR-QX(S1).Cryosections were stained with monoclonal antibodies to detect CSF-1R+ (red) and CD8β+ (green) cells. Nuclei were labeled with DAPI (blue). Scale bars, 50μm. Representative images are shown in all panels.
From: Ellis S, Keep S, Britton P, de Wit S, Bickerton E, Vervelde L.
Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In Vivo.
J Virol. 2018 Nov 12;92(23):e01473-18.
doi: 10.1128/JVI.01473-18
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF ResetMouse anti Chicken CD8 Beta
- Product Type
- Monoclonal Antibody
- Clone
- EP42
- Isotype
- IgG2a
- Specificity
- CD8 Beta
Antibody Quality and 
Unrivaled 
| Mouse anti Chicken CD8 beta antibody, clone EP42 recognises Chicken CD8β. CD8 is a dimer formed of either two alpha chains, or an alpha chain and a beta chain.Mouse anti Chicken CD8 beta antibody, clone EP42 is specific for Chicken CD8β, binding to an epitope expressed on the CD8β chain, available only on cells expressing alpha/beta heterodimers. |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:FITC | ||||||
References for CD8 Beta antibody
-
Tregaskes, C.A .et al. (1995) Identification and analysis of the expression of CD8 alpha beta and CD8 alpha alpha isoforms in chickens reveals a major TCR-gamma delta CD8 alpha beta subset of intestinal intraepithelial lymphocytes.
J Immunol. 154 (9): 4485-94. -
Imhof, B.A. et al. (2000) Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires.
J Immunol. 165: 6716-22. -
Katevuo, K. and Vainio, O. (1996) Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery.
Immunology. 89: 419-23. -
Luhtala, M. et al. (1995) Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes.
Scand J Immunol. 42 (1): 171-4. -
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8 alpha molecule.
Poult Sci. 78 (11): 1526-31. -
Paramithiotis E et al. (1991) High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes.
J Immunol. 147 (11): 3710-7. -
Ellis, S. et al. (2018) Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In Vivo.
J Virol.92 (23): e01473-18.
- RRID
- AB_10717852
- UniProt
- Q90769
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