CD8 Beta antibody | EP42
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Mouse anti Chicken CD8 beta antibody, clone EP42 recognises Chicken CD8β. CD8 is a dimer formed of either two alpha chains, or an alpha chain and a beta chain.Mouse anti Chicken CD8 beta antibody, clone EP42 is specific for Chicken CD8β, binding to an epitope expressed on the CD8β chain, available only on cells expressing alpha/beta heterodimers.
Product Details
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q90769
- Regulatory
- For research purposes only
Applications of CD8 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
|
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| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | C E WB | 0.5 mg |
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| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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Product Specific References
References for CD8 Beta antibody
-
Tregaskes, C.A .et al. (1995) Identification and analysis of the expression of CD8 alpha beta and CD8 alpha alpha isoforms in chickens reveals a major TCR-gamma delta CD8 alpha beta subset of intestinal intraepithelial lymphocytes.
J Immunol. 154 (9): 4485-94. -
Imhof, B.A. et al. (2000) Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires.
J Immunol. 165: 6716-22. -
Katevuo, K. and Vainio, O. (1996) Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery.
Immunology. 89: 419-23. -
Luhtala, M. et al. (1995) Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes.
Scand J Immunol. 42 (1): 171-4. -
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8 alpha molecule.
Poult Sci. 78 (11): 1526-31. -
Paramithiotis E et al. (1991) High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes.
J Immunol. 147 (11): 3710-7.
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