CD8 Beta antibody | EP42

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Anti Chicken CD8 Beta Antibody, clone EP42 thumbnail image 1

Anti Chicken CD8 Beta Antibody, clone EP42 gallery image 1 — **Published customer image:**
Mouse anti Chicken CD8 beta antibody, clone EP42 (**MCA5763** used to label CD8b cells in chickin conjunctiva associated lymphoid tissue by immunofluorescence.
**Image caption:**
Cellular infiltrates of head-associated lymphoid tissues following vaccination with BeauR-M41(S1) and BeauR-QX(S1).Cryosections were stained with monoclonal antibodies to detect CSF-1R+ (red) and CD8β+ (green) cells. Nuclei were labeled with DAPI (blue). Scale bars, 50μm. Representative images are shown in all panels.

From: Ellis S, Keep S, Britton P, de Wit S, Bickerton E, Vervelde L.
Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication *In Vivo*.
J Virol. 2018 Nov 12;92(23):e01473-18.
doi: 10.1128/JVI.01473-18
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Chicken CD8 Beta

Product Type: Monoclonal Antibody
Clone: EP42
Isotype: IgG2a
Specificity: CD8 Beta

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Secondary Antibodies
References (7)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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MCA5763 Datasheet Datasheet SDS SDS	C F IP	0.25 mg		Log in	Get Quote
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Mouse anti Chicken CD8 beta antibody, clone EP42 recognises Chicken CD8β. CD8 is a dimer formed of either two alpha chains, or an alpha chain and a beta chain.Mouse anti Chicken CD8 beta antibody, clone EP42 is specific for Chicken CD8β, binding to an epitope expressed on the CD8β chain, available only on cells expressing alpha/beta heterodimers.

Target Species: Chicken
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution: Borate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Approx. Protein Concentrations: IgG concentration 0.5mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

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References for CD8 Beta antibody

Tregaskes, C.A .et al. (1995) Identification and analysis of the expression of CD8 alpha beta and CD8 alpha alpha isoforms in chickens reveals a major TCR-gamma delta CD8 alpha beta subset of intestinal intraepithelial lymphocytes.
J Immunol. 154 (9): 4485-94.
Imhof, B.A. et al. (2000) Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires.
J Immunol. 165: 6716-22.
Katevuo, K. and Vainio, O. (1996) Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery.
Immunology. 89: 419-23.
Luhtala, M. et al. (1995) Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes.
Scand J Immunol. 42 (1): 171-4.
Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8 alpha molecule.
Poult Sci. 78 (11): 1526-31.
Paramithiotis E et al. (1991) High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes.
J Immunol. 147 (11): 3710-7.
Ellis, S. et al. (2018) Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In Vivo.
J Virol.92 (23): e01473-18.