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CD4 antibody | 2-35

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Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 1 Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 2 Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 3 Anti Chicken CD4 Antibody, clone 2-35 thumbnail image 4
Anti Chicken CD4 Antibody, clone 2-35 gallery image 1
Figure A. RPE conjugated Mouse anti Chicken CD8 (MCA2166PE) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. PE conjugated Mouse anti Chicken CD8 (MCA2166PE) and FITC conjugated Mouse anti Chicken CD4 (MCA2164F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Chicken CD4 Antibody, clone 2-35 gallery image 2
Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and RPE conjugated Mouse anti Chicken CD4 (MCA2164PE). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Chicken CD4 Antibody, clone 2-35 gallery image 3
Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and Mouse IgG2b isotype control (MCA691) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A). Figure B. Mouse anti Chicken CD4 (MCA2164GA) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A) and FITC conjugated Mouse anti Chicken CD8 (MCA2166F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Chicken CD4 Antibody, clone 2-35 gallery image 4
Published customer image:
FITC conjugated Mouse anti Chicken CD4 antibody, clone 2-35 (MCA2164F) used to label turkey lymphocytes by flow cytometry.
Image caption:
Gating strategy for extracellular staining for CD4+, CD8α+, CD4+CD8α+ and IgM+ cells in spleen sample of the examined turkeys. Abbreviations; FSC-A: forward scatter area, FSC-H: forward scatter height SSC-A: side scatter area.

From: Konieczka P, Tykałowski B, Ognik K, Kinsner M, Szkopek D, Wójcik M, Mikulski D, Jankowski J.
Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.
Vet Res. 2022 Jul 26;53(1):59.
doi: 10.1186/s13567-022-01080-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Chicken CD4

Product Type
Monoclonal Antibody
Clone
2-35
Isotype
IgG2b
Specificity
CD4

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Product Code Applications Pack Size List Price Your Price Qty
MCA2164GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
List Price Your Price
loader

Mouse anti Chicken CD4, clone 2-35 recognizes the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells (Luhtala et al. 1993). Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4 (Li et al. 1998).

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Chicken embryonic thymocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Lymph node cells from immunized Balb/c mice were fused with cells of the SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
List Price Your Price
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for CD4 antibody

  1. Vainio, O. et al. (1989) Characterization of chicken CD4-expressing cells.
    Prog Clin Biol Res. 307: 45-56.
  2. Luhtala, M. et al. (1993) Analysis of chicken CD4 by monoclonal antibodies indicates evolutionary conservation between avian and mammalian species.
    Hybridoma. 12: 633-46.
  3. Koskinen, R. et al. (1999) Cloning and modeling of the first nonmammalian CD4.
    J Immunol. 162 (7): 4115-21.
  4. Li, Z. et al. (1999) Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8alpha molecule.
    Poult Sci. 78 (11): 1526-31.
  5. Pavlova, S.P. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
    J Gen Virol. 91 (Pt 4): 847-57.
  6. Rosa, A.C. et al. (2014) Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults.
    Arch Virol. 159 (6): 1453-7.
  7. Sachan, S. et al. (2015) Adjuvant potential of resiquimod with inactivated Newcastle disease vaccine and its mechanism of action in chicken.
    Vaccine. 33 (36): 4526-32.
  8. Blohm, U. et al. (2016) Immunological Competence of Different Domestic Chicken Breeds Against Avian Influenza Infection.
    Avian Dis. 60 (1 Suppl): 262-8.
    9. View The Latest Product References
  9. Kannan, T.A. et al. (2017) Age Related Changes in T Cell Subsets in Thymus and Spleen of Layer Chicken (Gallus domesticus)
    Int J Curr Microbiol App Sci. 6 (1): 15-19.
  10. Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers.
    Poult Sci. 96 (7): 2064-73.
  11. Tang, Y. et al. (2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?
    Pathogens. 9 (10): 843.
  12. Konieczka, P. et al. (2022) Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.
    Vet Res. 53 (1): 59.
  13. Hohensee, L. et al. (2024) The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens.
    Vet Res. 55 (1): 5.
  14. Song, J. et al. (2022) GPR15-C10ORF99 functional pairing initiates colonic Treg homing in amniotes.
    EMBO Rep. 23 (3): e53246.
  15. Härtle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
    Frontiers in Veterinary Science. 23 May [Epub ahead of print].

MCA2164GA

161440 1703

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