CD3 antibody | CT-3

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Mouse anti Chicken CD3

CD3 antibody, clone CT-3, recognizes the chicken CD3 and is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.

Product Type
Monoclonal Antibody
Clone
CT-3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1473 C F IP 0.5 mg
Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.

In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.

Product Details

Target Species
Chicken
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared from ascites
Buffer Solution
Borate buffered saline
Preservative Stabilisers
None present
Immunogen
Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Lymph node cells from immunised Balb/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
Q98910 Related reagents
Entrez Gene
CD3E Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
Regulatory
For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for CD3 antibody

  1. Chen, C.L. et al. (1986) Identification of a T3/T cell receptor complex in chickens.
    J Exp Med. 164 (1): 375-80.
  2. Mukamoto, M. & Kodama, H. (2000) Regulation of early chicken thymocyte proliferation by transforming growth factor-beta from thymic stromal cells and thymocytes.
    Vet Immunol Immunopathol. 77 (1-2): 121-32.
  3. Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.
    Infect Immun. 79: 3445-54.
  4. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  5. Abd El-Hack, M.. & Alagawany, M. (2015) Performance, egg quality, blood profile, immune function, and antioxidant enzyme activities in laying hens fed diets with thyme powder
    Journal of Animal and Feed Sciences. 24 (2): 127-33.

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