CD3 antibody | CT-3
Mouse anti Chicken CD3
CD3 antibody, clone CT-3, recognizes the chicken CD3 and is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
- Product Type
- Monoclonal Antibody
- Clone
- CT-3
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1473 | C F IP | 0.5 mg |
|
Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.
In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.
Product Details
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Lymph node cells from immunised Balb/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- Q98910 Related reagents
- Entrez Gene
- CD3E Related reagents
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- Regulatory
- For research purposes only
Applications of CD3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | ||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
|
|
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
|
|
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
|
Product Specific References
References for CD3 antibody
-
Chen, C.L. et al. (1986) Identification of a T3/T cell receptor complex in chickens.
J Exp Med. 164 (1): 375-80. -
Mukamoto, M. & Kodama, H. (2000) Regulation of early chicken thymocyte proliferation by transforming growth factor-beta from thymic stromal cells and thymocytes.
Vet Immunol Immunopathol. 77 (1-2): 121-32. -
Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.
Infect Immun. 79: 3445-54. -
Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 141: 3255-65.
