CD3 antibody | CT-3



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F ResetMouse anti Chicken CD3
- Product Type
- Monoclonal Antibody
- Clone
- CT-3
- Isotype
- IgG1
- Specificity
- CD3
Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa. In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow. |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Lymph node cells from immunised Balb/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | |
Immunohistology - Frozen | ![]() |
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Immunoprecipitation | ![]() |
- Flow Cytometry
- Use 50μl of the suggested working dilution to label 106 cells in 100μl
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Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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Description | Rabbit F(ab')2 anti Mouse IgG:FITC |
References for CD3 antibody
-
Chen, C.L. et al. (1986) Identification of a T3/T cell receptor complex in chickens.
J Exp Med. 164 (1): 375-80. -
Mukamoto, M. & Kodama, H. (2000) Regulation of early chicken thymocyte proliferation by transforming growth factor-beta from thymic stromal cells and thymocytes.
Vet Immunol Immunopathol. 77 (1-2): 121-32. -
Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.
Infect Immun. 79: 3445-54. -
Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Development. 141: 3255-65. -
Tang, Y. et al. (2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?
Pathogens. 9(10):843.
- RRID
- AB_321244
- UniProt
- Q98910
- Entrez Gene
- CD3E
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
MCA1473


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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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