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CD3 antibody | CT-3

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Anti Chicken CD3 Antibody, clone CT-3 thumbnail image 1 Anti Chicken CD3 Antibody, clone CT-3 thumbnail image 2 Anti Chicken CD3 Antibody, clone CT-3 thumbnail image 3
Anti Chicken CD3 Antibody, clone CT-3 gallery image 1
Figure A. RPE conjugated Mouse anti Chicken CD8 (MCA2166PE) and Mouse IgG1 isotype control (MCA928) conjugated with Goat anti Mouse IgG (H/L):FITC (STAR117F). Figure B. Mouse anti Chicken CD3 (MCA1473) conjugated with Goat anti Mouse IgG (H/L):FITC (STAR117F) and RPE conjugated Mouse anti Chicken CD8 (MCA2166PE). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Chicken CD3 Antibody, clone CT-3 gallery image 2
Figure A. FITC conjugated Mouse anti Chicken CD4 (MCA2164F) and Mouse IgG1 isotype control (MCA928) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A). Figure B. Mouse anti Chicken CD3 (MCA1473) followed with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A) and FITC conjugated Mouse anti Chicken CD4 (MCA2164F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Chicken CD3 Antibody, clone CT-3 gallery image 3
Figure A. FITC conjugated Mouse anti Chicken CD8 (MCA2166F) and Mouse IgG1 isotype control (MCA928) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A). Figure B. Mouse anti Chicken CD3 (MCA1473) followed with Rabbit F(ab')2 anti Mouse IgG:RPE (STAR12A) and FITC conjugated Mouse anti Chicken CD8 (MCA2166F). All experiments performed on peripheral blood mononuclear cells extracted from whole chicken blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Chicken CD3

Product Type
Monoclonal Antibody
Clone
CT-3
Isotype
IgG1
Specificity
CD3

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Product Code Applications Pack Size List Price Your Price Qty
MCA1473
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.5 mg
List Price Your Price

Mouse anti Chicken CD3, clone CT-3 recognizes the chicken CD3 cell surface antigen expressed by T cells (Chen et al. 1986). The antibody also co-precipitates a ~90 kDa heterodimer from digitonin solubilised T cell lysates, which can be reduced to two polypeptides of ~40 kDa and ~50 kDa.

In the chicken, CD3 is expressed by approximately 30% of thymocytes, 70% of peripheral blood mononuclear cells, but <1% of cells in the bursa and bone marrow.

Target Species
Chicken
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared from ascites
Buffer Solution
Borate buffered saline
Preservative Stabilisers
None present
Immunogen
Chicken thymocytes and Ig -ve peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Lymph node cells from immunised Balb/c mice were fused with cells from the mouse P3-X63Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg
List Price Your Price
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml
List Price Your Price
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for CD3 antibody

  1. Chen, C.L. et al. (1986) Identification of a T3/T cell receptor complex in chickens.
    J Exp Med. 164 (1): 375-80.
  2. Mukamoto, M. & Kodama, H. (2000) Regulation of early chicken thymocyte proliferation by transforming growth factor-beta from thymic stromal cells and thymocytes.
    Vet Immunol Immunopathol. 77 (1-2): 121-32.
  3. Chaussé, A.M. et al. (2011) Expression of toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to salmonella carrier state.
    Infect Immun. 79: 3445-54.
  4. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development. 141: 3255-65.
  5. Tang, Y. et al. (2020) Immune Modulation and the Development of Fowl Typhoid: A Model of Human Disease?
    Pathogens. 9(10):843.

RRID
AB_321244
UniProt
Q98910
Entrez Gene
CD3E
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity

MCA1473

159172 161752

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