CD25 antibody | AV142
(Image courtesy of the Roslin Institute).
Mouse anti Chicken CD25
- Product Type
- Monoclonal Antibody
- Clone
- AV142
- Isotype
- IgG1
- Specificity
- CD25
Antibody Quality and Performance Guarantee
| Mouse anti Chicken CD25 monoclonal antibody, clone AV142 recognizes CD25, the chicken IL-2 Receptor alpha chain (IL-2Rα) which has been identified as the chicken homologue of human CD25 and is cited as ChCD25 in published literature. Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes. CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection . Chicken IL-2Rα is the low affinity receptor for Interleukin-2. Mouse anti Chicken CD25 monoclonal antibody, clone AV142 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to this anti chicken CD25 antibody, other antibodies to chicken CD antigens and cytokines from this project are now available from Bio-Rad. |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Chicken CD25/huIg fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 Ag14 Myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This reagent was produced by the WellcomeTrust-funded National Avian Resource Facility (Grant number WT099164MA).
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- ELISA
- This product is suitable for use in direct ELISA applications.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human anti Chicken CD25 | HCA173 | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Human anti Chicken CD25 | ||||||
| Mouse anti Chicken CD4:FITC | MCA2164F | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Chicken CD4:FITC | ||||||
| Mouse anti Chicken CD4:RPE | MCA2164PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Chicken CD4:RPE | ||||||
| Mouse anti Chicken CD8 Alpha:FITC | MCA2166F | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Chicken CD8 Alpha:FITC | ||||||
| Mouse anti Chicken CD8 Alpha:RPE | MCA2166PE | F | 100 Tests/1ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Chicken CD8 Alpha:RPE | ||||||
References for CD25 antibody
-
Neulen, M.L. et al. (2015) Identification of novel chicken CD4+ CD3- blood population with NK cell like features.
Dev Comp Immunol. 49 (1): 72-8. -
Geng, Y. et al. (2018) Dietary vitamin D(3) supplementation protects laying hens against lipopolysaccharide-induced immunological stress.
Nutr Metab (Lond). 15: 58. -
Hohensee, L. et al. (2024) The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens.
Vet Res. 55 (1): 5. -
Häätle, S. et al. (2024) Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Frontiers in Veterinary Science. 11 [Epub ahead of print].
- Synonyms
- IL-2R Alpha Chain
- UniProt
- Q90WJ4
MCA5925GA
152082
156842
159855
164554
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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