CD25 antibody | AV142

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Mouse anti Chicken CD25

Product Type
Monoclonal Antibody
Clone
AV142
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5925GA E F WB 0.1 mg
Mouse anti Chicken CD25 monoclonal antibody, clone AV142 recognizes CD25, the chicken IL-2 Receptor alpha chain (IL-2Rα) which has been identified as the chicken homologue of human CD25 and is cited as ChCD25 in published literature.
Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection .
Chicken IL-2Rα is the low affinity receptor for Interleukin-2.

Mouse anti Chicken CD25 monoclonal antibody, clone AV142 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to this anti chicken CD25 antibody, other antibodies to chicken CD antigens and cytokines from this project are now available from Bio-Rad.

Product Details

Target Species
Chicken
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Chicken CD25/huIg fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 Ag14 Myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q90WJ4 Related reagents
Acknowledgements
This reagent was produced by the WellcomeTrust-funded National Avian Resource Facility (Grant number WT099164MA).
Regulatory
For research purposes only

Applications of CD25 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
ELISA
This product is suitable for use in direct ELISA applications.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L) (Multi Species Adsorbed) STAR117 0.5 mg E F WB
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed) STAR117D405GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Chicken CD25 HCA173 0.1 mg F
Mouse anti Chicken CD4:FITC MCA2164F 0.1 mg F
Mouse anti Chicken CD4:RPE MCA2164PE 100 Tests F
Mouse anti Chicken CD8 Alpha:FITC MCA2166F 0.1 mg F
Mouse anti Chicken CD8 Alpha:RPE MCA2166PE 0.1 mg F

Product Specific References

References for CD25 antibody

  1. Neulen, M.L. et al. (2015) Identification of novel chicken CD4+ CD3- blood population with NK cell like features.
    Dev Comp Immunol. 49 (1): 72-8.