Cetuximab antibody | AbD19830_hIgG1

Human anti Cetuximab

Product Type
Monoclonal Antibody
Clone
AbD19830_hIgG1
Isotype
IgG1
Specificity
Cetuximab

Product Code Applications Pack Size List Price Your Price Qty
HCA221
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Human anti Cetuximab, clone AbD19830_hIgG1, is an anti-idiotypic antibody that binds specifically to cetuximab and inhibits the binding of the drug to its target. Human anti Cetuximab, clone AbD19830_hIgG1 can be used to measure the levels of cetuximab and biosimilar products using bioanalytical assays.

In the HRP-conjugated format clone AbD19830_hIgG1 is ideal for use as detection antibody in pharmacokinetic (PK) assays. It is a fully human antibody and therefore may also be used as a calibrator in immune response assays to measure the anti-drug-antibody (ADA) response in patient sera.

Cetuximab (brand name Erbitux®) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody, directed against the Epidermal Growth Factor Receptor (EGFR), inhibits tumor cell proliferation by blocking the interaction of Epidermal Growth Factor (EGF) with its receptor.

View a summary of all anti cetuximab antibodies.

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Cetuximab.
Affinity
The monovalent intrinsic affinity of Human anti Cetuximab antibody, clone AbD19830_hIgG1 is KD=1.7 nM as measured by real time, label free molecular interaction analysis on immobilized Cetuximab.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used in a direct ELISA or as a bridging reagent in ADA assays. Following HRP-conjugation, this product can also be used as a detection reagent in a bridging ELISA for PK assay development together with HCA220 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug.
Protocol: ADA bridging ELISA.

Description Product Code Applications Pack Size List Price Your Price Quantity
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Cetuximab HCA220 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Cetuximab

Further Reading

  1. Gomez, D. et al. (2013) Cetuximab therapy in the treatment of metastatic colorectal cancer: the future frontier?
    Int J Surg. 11 (7): 507-13.

Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Cetuximab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"