CCT eta antibody | PK/16/8/a
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
CCT-eta but not CCT-beta protein and mRNA are differentially expressed in fetal versus adult fibroblasts. RNA and protein extracted from fetal and adult fibroblasts were subjected to qRT PCR (a & b) and Western blot (c & d) analyses respectively. CCT-eta mRNA was significantly more abundant in adult fibroblasts when compared to fetal fibroblasts (a); there was no significant difference in CCT-beta message levels between fetal and adult fibroblasts (b). Values are means ± SEM of three independent studies performed in duplicate. Statistical analyses were performed using Student's t test. NS = non-significant. Equal amounts of protein loaded from fetal and adult fibroblasts showed that adult fibroblasts express significantly greater CCT-eta protein(c). In contrast CCT-beta protein levels were not different between fetal and adult fibroblasts (d). Blots shown here are representative of at least three different experiments.
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility.
PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
siRNA downregulation of CCT-eta but not CCT-beta reduces α-SMA protein levels in adult fibroblasts. (a & b)Western blot showed effective reduction of both CCT-eta and α-SMA protein levels when CCT-eta siRNA was administered, leaving beta-actin largely unaffected. In contrast, downregulating CCT-beta did not lead to significant reduction in either α-SMA or beta-actin levels. GAPDH was used as loading internal control. A representative immunoblot of up to three similar such blots are shown for each analysis. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA.
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCTeta antibody, clone PK/16/8/a recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCTη is a 543 amino acid ~60 kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1)
The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60 kDa in HeLa cell lysates using western blotting under reducing conditions.
The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60 kDa in HeLa cell lysates using western blotting under reducing conditions.
Product Details
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Human Bovine Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q2NKZ1
- Entrez Gene
- CCT7
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0006457 protein folding
- GO:0005524 ATP binding
- Regulatory
- For research purposes only
Applications of CCT eta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 1/100 | ||
| Western Blotting | 1/500 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human prostate.
- Western Blotting
- MCA2179 detects a band of approximately 60kD in Hela cell lysates.
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| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Product Specific References
References for CCT eta antibody
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Satish, L. et al. (2010) Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds.
Cell Stress Chaperones. 15 (6): 819-26. -
Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
Electrophoresis. 17 (11): 1720-7. -
Satish L et al. (2011) Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.
Cell Stress Chaperones. 16 (6): 675-80. -
Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
J Biol Chem. 275 (25): 18985-94. -
Satish, L. et al. (2010) Chaperonin containing T-complex polypeptide subunit eta (CCT-eta) is a specific regulator of fibroblast motility and contractility.
PLoS One. 5(4):e10063. -
Satish L et al. (2013) Increased CCT-eta expression is a marker of latent and active disease and a modulator of fibroblast contractility in Dupuytren's contracture.
Cell Stress Chaperones. 18 (4): 397-404.
Further Reading
-
Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
EMBO J. 19 (22): 5971-9.
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