CCT eta antibody | PK/16/8/a
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
CCT-eta but not CCT-beta protein and mRNA are differentially expressed in fetal versus adult fibroblasts. RNA and protein extracted from fetal and adult fibroblasts were subjected to qRT PCR (a & b) and Western blot (c & d) analyses respectively. CCT-eta mRNA was significantly more abundant in adult fibroblasts when compared to fetal fibroblasts (a); there was no significant difference in CCT-beta message levels between fetal and adult fibroblasts (b). Values are means ± SEM of three independent studies performed in duplicate. Statistical analyses were performed using Student's t test. NS = non-significant. Equal amounts of protein loaded from fetal and adult fibroblasts showed that adult fibroblasts express significantly greater CCT-eta protein(c). In contrast CCT-beta protein levels were not different between fetal and adult fibroblasts (d). Blots shown here are representative of at least three different experiments.
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility.
PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
siRNA downregulation of CCT-eta but not CCT-beta reduces α-SMA protein levels in adult fibroblasts. (a & b)Western blot showed effective reduction of both CCT-eta and α-SMA protein levels when CCT-eta siRNA was administered, leaving beta-actin largely unaffected. In contrast, downregulating CCT-beta did not lead to significant reduction in either α-SMA or beta-actin levels. GAPDH was used as loading internal control. A representative immunoblot of up to three similar such blots are shown for each analysis. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA.
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB ResetRat anti CCT eta
- Product Type
- Monoclonal Antibody
- Clone
- PK/16/8/a
- Isotype
- IgG2a
- Specificity
- CCT eta
Antibody Quality and Performance Guarantee
| Rat anti CCTeta antibody, clone PK/16/8/a recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCTη is a 543 amino acid ~60 kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1) The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein. Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60 kDa in HeLa cell lysates using western blotting under reducing conditions. |
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Human Bovine Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
1/100 | |
| Western Blotting | |
1/500 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human prostate
- Western Blotting
- Rat anti CCT eta antibody, clone PK/16/8/a detects a band of approximately 60 kDa in Hela cell lysates
References for CCT eta antibody
-
Satish, L. et al. (2010) Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds.
Cell Stress Chaperones. 15 (6): 819-26. -
Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
Electrophoresis. 17 (11): 1720-7. -
Satish L et al. (2011) Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.
Cell Stress Chaperones. 16 (6): 675-80. -
Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
J Biol Chem. 275 (25): 18985-94. -
Satish, L. et al. (2010) Chaperonin containing T-complex polypeptide subunit eta (CCT-eta) is a specific regulator of fibroblast motility and contractility.
PLoS One. 5(4):e10063. -
Satish L et al. (2013) Increased CCT-eta expression is a marker of latent and active disease and a modulator of fibroblast contractility in Dupuytren's contracture.
Cell Stress Chaperones. 18 (4): 397-404.
Further Reading
-
Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
EMBO J. 19 (22): 5971-9.
- RRID
- AB_324836
- UniProt
- Q2NKZ1
- Entrez Gene
- CCT7
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0006457 protein folding
- GO:0005524 ATP binding
MCA2179
150117
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CCT eta specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up