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CCT eta antibody | PK/16/8/a

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Anti CCT eta Antibody, clone PK/16/8/a thumbnail image 1 Anti CCT eta Antibody, clone PK/16/8/a thumbnail image 2 Anti CCT eta Antibody, clone PK/16/8/a thumbnail image 3 Anti CCT eta Antibody, clone PK/16/8/a thumbnail image 4
Anti CCT eta Antibody, clone PK/16/8/a gallery image 1
Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell lysate (2) probed with Rat anti CCT eta (MCA2179) followed by F(ab')2 Rabbit anti Rat IgG conjugated to HRP and visualized by chemiluminescence.
Anti CCT eta Antibody, clone PK/16/8/a gallery image 2
Published customer image:
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
CCT-eta but not CCT-beta protein and mRNA are differentially expressed in fetal versus adult fibroblasts. RNA and protein extracted from fetal and adult fibroblasts were subjected to qRT PCR (a & b) and Western blot (c & d) analyses respectively. CCT-eta mRNA was significantly more abundant in adult fibroblasts when compared to fetal fibroblasts (a); there was no significant difference in CCT-beta message levels between fetal and adult fibroblasts (b). Values are means ± SEM of three independent studies performed in duplicate. Statistical analyses were performed using Student's t test. NS = non-significant. Equal amounts of protein loaded from fetal and adult fibroblasts showed that adult fibroblasts express significantly greater CCT-eta protein(c). In contrast CCT-beta protein levels were not different between fetal and adult fibroblasts (d). Blots shown here are representative of at least three different experiments.

From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility.
PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti CCT eta Antibody, clone PK/16/8/a gallery image 3
Published customer image:
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
siRNA downregulation of CCT-eta but not CCT-beta reduces α-SMA protein levels in adult fibroblasts. (a & b)Western blot showed effective reduction of both CCT-eta and α-SMA protein levels when CCT-eta siRNA was administered, leaving beta-actin largely unaffected. In contrast, downregulating CCT-beta did not lead to significant reduction in either α-SMA or beta-actin levels. GAPDH was used as loading internal control. A representative immunoblot of up to three similar such blots are shown for each analysis. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA.

From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti CCT eta Antibody, clone PK/16/8/a gallery image 4
Published customer image:
Rat anti CCTη antibody, clone PK/16/8/a (MCA2179) used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:(c & d) Western blot results using CCT-eta and CCT-beta antibody (1∶500) showed effective reduction of CCT-eta and CCT-beta protein levels when siRNA was administered but no decrease when scrambled siRNA was employed. GAPDH was used as a loading control. A representative immunoblot of up to four similar such blots is shown for each analysis.

From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010)
Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.This is from an open access article distributed under terms of a Creative Commons Attribution License.

Rat anti CCT eta

Product Type
Monoclonal Antibody
Clone
PK/16/8/a
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2179
Datasheet
P* WB
SDS
0.1 mg loader

Rat anti CCTeta antibody, clone PK/16/8/a recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCTη is a 543 amino acid ~60 kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1)

The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.

Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60 kDa in HeLa cell lysates using western blotting under reducing conditions.

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
Bovine
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q2NKZ1
Entrez Gene
CCT7
GO Terms
GO:0051082 unfolded protein binding
GO:0006457 protein folding
GO:0005524 ATP binding
Regulatory
For research purposes only

Applications of CCT eta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/100
Western Blotting 1/500
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human prostate.
Western Blotting
MCA2179 detects a band of approximately 60kD in Hela cell lysates.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Application Based External Images

Western Blotting

Product Specific References

References for CCT eta antibody

  1. Satish, L. et al. (2010) Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds.
    Cell Stress Chaperones. 15 (6): 819-26.
  2. Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
    Electrophoresis. 17 (11): 1720-7.
  3. Satish L et al. (2011) Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.
    Cell Stress Chaperones. 16 (6): 675-80.
  4. Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
    J Biol Chem. 275 (25): 18985-94.
  5. Satish, L. et al. (2010) Chaperonin containing T-complex polypeptide subunit eta (CCT-eta) is a specific regulator of fibroblast motility and contractility.
    PLoS One. 5(4):e10063.
  6. Satish L et al. (2013) Increased CCT-eta expression is a marker of latent and active disease and a modulator of fibroblast contractility in Dupuytren's contracture.
    Cell Stress Chaperones. 18 (4): 397-404.

Further Reading

  1. Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
    EMBO J. 19 (22): 5971-9.

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