CCT eta antibody | PK/16/8/a




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WB ResetRat anti CCT eta
- Product Type
- Monoclonal Antibody
- Clone
- PK/16/8/a
- Isotype
- IgG2a
- Specificity
- CCT eta
Rat anti CCTeta antibody, clone PK/16/8/a recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCTη is a 543 amino acid ~60 kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1) The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein. Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60 kDa in HeLa cell lysates using western blotting under reducing conditions. |
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Human Bovine Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin 1 | ![]() |
1/100 | |
Western Blotting | ![]() |
1/500 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
- Human prostate
- Western Blotting
- Rat anti CCT eta antibody, clone PK/16/8/a detects a band of approximately 60 kDa in Hela cell lysates
References for CCT eta antibody
-
Satish, L. et al. (2010) Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds.
Cell Stress Chaperones. 15 (6): 819-26. -
Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
Electrophoresis. 17 (11): 1720-7. -
Satish L et al. (2011) Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.
Cell Stress Chaperones. 16 (6): 675-80. -
Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
J Biol Chem. 275 (25): 18985-94. -
Satish, L. et al. (2010) Chaperonin containing T-complex polypeptide subunit eta (CCT-eta) is a specific regulator of fibroblast motility and contractility.
PLoS One. 5(4):e10063. -
Satish L et al. (2013) Increased CCT-eta expression is a marker of latent and active disease and a modulator of fibroblast contractility in Dupuytren's contracture.
Cell Stress Chaperones. 18 (4): 397-404.
Further Reading
-
Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
EMBO J. 19 (22): 5971-9.
- RRID
- AB_324836
- UniProt
- Q2NKZ1
- Entrez Gene
- CCT7
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0006457 protein folding
- GO:0005524 ATP binding
MCA2179

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