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CCT Epsilon antibody | PK/29/23/8d

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Rat anti CCT Epsilon

Product Type
Monoclonal Antibody
Clone
PK/29/23/8d
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2178
Datasheet
GS IP WB
SDS
0.1 mg loader

Rat anti CCT epsilon antibody, clone PK/29/23/8d recognizes the epsilon polypeptide of the CCT chaperonin molecule complex. CCT epsilon, also known as T-complex protein 1 subunit epsilon, CCT5 or CCTε is a 540 amino acid ~60 kDa molecular chaperone. The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.

Two isoforms of CCTε produced by alternative splicing have been described. Rat anti CCT epsilon antibody, clone PK/29/23/8d binds to the canonical isoform 1, binding to the truncated isoform 2 has not been evaluated (UniProt : P48643).

Mutations in the CCT5 gene can lead to the development of Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive (HSNSP) a disease characterized by spastic paraplegia and progressive neuropathy with limb ulceration (Bouhouche et al. 2006).

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rabbit
Rat
Human
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P48643
Entrez Gene
CCT5
GO Terms
GO:0051082 unfolded protein binding
GO:0009615 response to virus
GO:0005524 ATP binding
GO:0005730 nucleolus
GO:0005815 microtubule organizing center
GO:0051084 'de novo' posttranslational protein folding
Regulatory
For research purposes only

Applications of CCT Epsilon antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Gel Super Shift Assays
Immunoprecipitation
Western Blotting 1/250 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2178 detects a band of approximately 60kD in lysates of heat shocked Hela cells.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Application Based External Images

Western Blotting

Product Specific References

References for CCT Epsilon antibody

  1. Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
    Electrophoresis. 17 (11): 1720-7.
  2. Howlett, A.C. et al. (2009) Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity.
    J Biol Chem. 284 (24): 16386-99.
  3. Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
    J Biol Chem. 275 (25): 18985-94.
  4. Hara, T. et al. (2007) Mass spectrometry analysis of the native protein complex containing actinin-4 in prostate cancer cells.
    Mol Cell Proteomics. 6 (3): 479-91.
  5. Tracy, C.M. et al. (2014) Programmed Cell Death Protein 5 Interacts with the Cytosolic Chaperonin Containing Tailless Complex Polypeptide 1 (CCT) to Regulate β-Tubulin Folding.
    J Biol Chem. 289: 4490-502.
  6. Gao, X. et al. (2013) Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins.
    J Biol Chem. 288 (36): 25760-8.
  7. Lai, C.W. et al. (2013) Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors.
    J Neurosci. 33 (18): 7941-51.

Further Reading

  1. Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
    EMBO J. 19 (22): 5971-9.

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