CCT Epsilon antibody | PK/29/23/8d
Two isoforms of CCTε produced by alternative splicing have been described. Rat anti CCT epsilon antibody, clone PK/29/23/8d binds to the canonical isoform 1, binding to the truncated isoform 2 has not been evaluated (UniProt : P48643).
Mutations in the CCT5 gene can lead to the development of Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive (HSNSP) a disease characterized by spastic paraplegia and progressive neuropathy with limb ulceration (Bouhouche et al. 2006).
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rabbit Rat Human Bovine
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0009615 response to virus
- GO:0005524 ATP binding
- GO:0005730 nucleolus
- GO:0005815 microtubule organizing center
- GO:0051084 'de novo' posttranslational protein folding
- For research purposes only
Applications of CCT Epsilon antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Gel Super Shift Assays|
- Western Blotting
- MCA2178 detects a band of approximately 60kD in lysates of heat shocked Hela cells.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)||STAR131A||C E P WB||1 ml|
|Goat anti Rat IgG:Biotin (Mouse Adsorbed)||STAR131B||C E IF P WB||0.5 mg|
|Rabbit F(ab')2 anti Rat IgG:Dylight®800||STAR16D800GA||F IF WB||0.1 mg|
|Rabbit F(ab')2 anti Rat IgG:FITC||STAR17B||F||1 mg|
|Rabbit F(ab')2 anti Rat IgG:HRP||STAR21B||C E P RE||1 mg|
|Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)||STAR69||F||0.5 ml|
|Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)||STAR71D800GA||F IF WB||0.1 mg|
|Goat anti Rat IgG:HRP (Mouse Adsorbed)||STAR72||C E P||0.5 mg|
|Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)||STAR73||F||0.5 ml|
Product Specific References
References for CCT Epsilon antibody
Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
Electrophoresis. 17 (11): 1720-7.
Howlett, A.C. et al. (2009) Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity.
J Biol Chem. 284 (24): 16386-99.
Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
J Biol Chem. 275 (25): 18985-94.
Hara, T. et al. (2007) Mass spectrometry analysis of the native protein complex containing actinin-4 in prostate cancer cells.
Mol Cell Proteomics. 6 (3): 479-91.
Tracy, C.M. et al. (2014) Programmed Cell Death Protein 5 Interacts with the Cytosolic Chaperonin Containing Tailless Complex Polypeptide 1 (CCT) to Regulate β-Tubulin Folding.
J Biol Chem. 289: 4490-502.
Gao, X. et al. (2013) Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins.
J Biol Chem. 288 (36): 25760-8.
Lai, C.W. et al. (2013) Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors.
J Neurosci. 33 (18): 7941-51.
Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
EMBO J. 19 (22): 5971-9.
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