CCT Epsilon antibody | PK/29/23/8d
Rat anti CCT epsilon antibody, clone PK/29/23/8d (MCA2178) used to immunoprecipitate protein complexes from transfected CHO-K1 cells.
Image caption:
Immunoprecipitation of CCT. CHO-K1 cells transfected with one of the WD40-V5 expressing constructs (TBL2-V5, MLST8-V5, or CDC20-V5) or with an empty vector as a control, were lysed and treated with anti-CCTε antibodies and protein A/G beads. The immunoprecipitation products and lysates from WD40-V5 transfected cells, serving as positive controls, were analyzed using immunoblotting with anti-V5 antibodies to identify the V5 tagged WD40 proteins
From: Mak WS, Tsang TM, Chan TY, Lukov GL.
Novel Binding Partners for CCT and PhLP1 Suggest a Common Folding Mechanism for WD40 Proteins with a 7-Bladed Beta-Propeller Structure.
Proteomes. 2021; 9(4):40.
doi: 10.3390/proteomes9040040
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCT epsilon antibody, clone PK/29/23/8d (MCA2178) used to detect CCTε in protein complexes from transfected CHO-K1 cells by western blotting
Image caption:
Immunoprecipitation of CCT. CHO-K1 cells transfected with one of the WD40-V5 expressing constructs (TBL2-V5, MLST8-V5, or CDC20-V5) or with an empty vector as a control, were lysed and treated with anti-CCTε antibodies and protein A/G beads. The immunoprecipitation products and lysates from WD40-V5 transfected cells, serving as positive controls, were analyzed using immunoblotting with anti-CCTε antibodies to identify CCT. Each panel shows a representative image of three repeats. The positive control for the CCT blot was a lysate from non-transfected cell.
From: Mak WS, Tsang TM, Chan TY, Lukov GL.
Novel Binding Partners for CCT and PhLP1 Suggest a Common Folding Mechanism for WD40 Proteins with a 7-Bladed Beta-Propeller Structure.
Proteomes. 2021; 9(4):40.
doi: 10.3390/proteomes9040040
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCT epsilon antibody, clone PK/29/23/8d recognizes the epsilon polypeptide of the CCT chaperonin molecule complex. CCT epsilon, also known as T-complex protein 1 subunit epsilon, CCT5 or CCTε is a 540 amino acid ~60 kDa molecular chaperone. The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
Two isoforms of CCTε produced by alternative splicing have been described. Rat anti CCT epsilon antibody, clone PK/29/23/8d binds to the canonical isoform 1, binding to the truncated isoform 2 has not been evaluated (UniProt : P48643).
Mutations in the CCT5 gene can lead to the development of Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive (HSNSP) a disease characterized by spastic paraplegia and progressive neuropathy with limb ulceration (Bouhouche et al. 2006).
Two isoforms of CCTε produced by alternative splicing have been described. Rat anti CCT epsilon antibody, clone PK/29/23/8d binds to the canonical isoform 1, binding to the truncated isoform 2 has not been evaluated (UniProt : P48643).
Mutations in the CCT5 gene can lead to the development of Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive (HSNSP) a disease characterized by spastic paraplegia and progressive neuropathy with limb ulceration (Bouhouche et al. 2006).
Product Details
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit Rat Human Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P48643
- Entrez Gene
- CCT5
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0009615 response to virus
- GO:0005524 ATP binding
- GO:0005730 nucleolus
- GO:0005815 microtubule organizing center
- GO:0051084 'de novo' posttranslational protein folding
- Regulatory
- For research purposes only
Applications of CCT Epsilon antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Gel Super Shift Assays | |||
| Immunoprecipitation | |||
| Western Blotting | 1/250 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2178 detects a band of approximately 60kD in lysates of heat shocked Hela cells.
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| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Product Specific References
References for CCT Epsilon antibody
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Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
Electrophoresis. 17 (11): 1720-7. -
Howlett, A.C. et al. (2009) Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity.
J Biol Chem. 284 (24): 16386-99. -
Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
J Biol Chem. 275 (25): 18985-94. -
Hara, T. et al. (2007) Mass spectrometry analysis of the native protein complex containing actinin-4 in prostate cancer cells.
Mol Cell Proteomics. 6 (3): 479-91. -
Tracy, C.M. et al. (2014) Programmed Cell Death Protein 5 Interacts with the Cytosolic Chaperonin Containing Tailless Complex Polypeptide 1 (CCT) to Regulate β-Tubulin Folding.
J Biol Chem. 289: 4490-502. -
Gao, X. et al. (2013) Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins.
J Biol Chem. 288 (36): 25760-8. -
Lai, C.W. et al. (2013) Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors.
J Neurosci. 33 (18): 7941-51. -
Mak, W.S. et al. (2021) Novel Binding Partners for CCT and PhLP1 Suggest a Common Folding Mechanism for WD40 Proteins with a 7-Bladed Beta-Propeller Structure
Proteomes 9(4): 40
Further Reading
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Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
EMBO J. 19 (22): 5971-9.
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