CCT Epsilon antibody | PK/29/23/8d
Rat anti CCT epsilon antibody, clone PK/29/23/8d (MCA2178) used to immunoprecipitate protein complexes from transfected CHO-K1 cells.
Image caption:
Immunoprecipitation of CCT. CHO-K1 cells transfected with one of the WD40-V5 expressing constructs (TBL2-V5, MLST8-V5, or CDC20-V5) or with an empty vector as a control, were lysed and treated with anti-CCTε antibodies and protein A/G beads. The immunoprecipitation products and lysates from WD40-V5 transfected cells, serving as positive controls, were analyzed using immunoblotting with anti-V5 antibodies to identify the V5 tagged WD40 proteins
From: Mak WS, Tsang TM, Chan TY, Lukov GL.
Novel Binding Partners for CCT and PhLP1 Suggest a Common Folding Mechanism for WD40 Proteins with a 7-Bladed Beta-Propeller Structure.
Proteomes. 2021; 9(4):40.
doi: 10.3390/proteomes9040040
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCT epsilon antibody, clone PK/29/23/8d (MCA2178) used to detect CCTε in protein complexes from transfected CHO-K1 cells by western blotting
Image caption:
Immunoprecipitation of CCT. CHO-K1 cells transfected with one of the WD40-V5 expressing constructs (TBL2-V5, MLST8-V5, or CDC20-V5) or with an empty vector as a control, were lysed and treated with anti-CCTε antibodies and protein A/G beads. The immunoprecipitation products and lysates from WD40-V5 transfected cells, serving as positive controls, were analyzed using immunoblotting with anti-CCTε antibodies to identify CCT. Each panel shows a representative image of three repeats. The positive control for the CCT blot was a lysate from non-transfected cell.
From: Mak WS, Tsang TM, Chan TY, Lukov GL.
Novel Binding Partners for CCT and PhLP1 Suggest a Common Folding Mechanism for WD40 Proteins with a 7-Bladed Beta-Propeller Structure.
Proteomes. 2021; 9(4):40.
doi: 10.3390/proteomes9040040
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti CCT Epsilon
- Product Type
- Monoclonal Antibody
- Clone
- PK/29/23/8d
- Isotype
- IgG2a
- Specificity
- CCT Epsilon
Antibody Quality and Performance Guarantee
| Rat anti CCT epsilon antibody, clone PK/29/23/8d recognizes the epsilon polypeptide of the CCT chaperonin molecule complex. CCT epsilon, also known as T-complex protein 1 subunit epsilon, CCT5 or CCTε is a 540 amino acid ~60 kDa molecular chaperone. The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein. Two isoforms of CCTε produced by alternative splicing have been described. Rat anti CCT epsilon antibody, clone PK/29/23/8d binds to the canonical isoform 1, binding to the truncated isoform 2 has not been evaluated (UniProt : P48643). Mutations in the CCT5 gene can lead to the development of Neuropathy, hereditary sensory, with spastic paraplegia, autosomal recessive (HSNSP) a disease characterized by spastic paraplegia and progressive neuropathy with limb ulceration (Bouhouche et al. 2006). |
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit Rat Human Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Gel Super Shift Assays |  |
||
| Immunoprecipitation | |
||
| Western Blotting | |
1/250 | 1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rat anti CCT epsilon antibody, clone PK/29/23/8d detects a band of approximately 60 kDa in lysates of heat shocked Hela cells.
References for CCT Epsilon antibody
-
Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: further characterisation of antibodies to individual subunits.
Electrophoresis. 17 (11): 1720-7. -
Hynes, G.M. & Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.
J Biol Chem. 275 (25): 18985-94. -
Hara, T. et al. (2007) Mass spectrometry analysis of the native protein complex containing actinin-4 in prostate cancer cells.
Mol Cell Proteomics. 6 (3): 479-91. -
Howlett, A.C. et al. (2009) Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity.
J Biol Chem. 284 (24): 16386-99. -
Lai, C.W. et al. (2013) Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors.
J Neurosci. 33 (18): 7941-51. -
Gao, X. et al. (2013) Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins.
J Biol Chem. 288 (36): 25760-8. -
Tracy, C.M. et al. (2014) Programmed Cell Death Protein 5 Interacts with the Cytosolic Chaperonin Containing Tailless Complex Polypeptide 1 (CCT) to Regulate β-Tubulin Folding.
J Biol Chem. 289: 4490-502. -
Mak, W.S. et al. (2021) Novel Binding Partners for CCT and PhLP1 Suggest a Common Folding Mechanism for WD40 Proteins with a 7-Bladed Beta-Propeller Structure
Proteomes 9(4): 40
View The Latest Product References
Further Reading
-
Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations.
EMBO J. 19 (22): 5971-9.
- Synonyms
- Tcp 1 Epsilon
- RRID
- AB_2073793
- UniProt
- P48643
- Entrez Gene
- CCT5
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0009615 response to virus
- GO:0005524 ATP binding
- GO:0005730 nucleolus
- GO:0005815 microtubule organizing center
- GO:0051084 'de novo' posttranslational protein folding
MCA2178
151256
161881
166388
181114
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