MHC Class II antibody | PF8J-9B
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Mouse anti Cat MHC class II antibody, clone PF8J-9B recognizes an epitope within the ~30 kDa beta chain region of cat MHC class II molecule, expressed by antigen presenting cells, B cells, monocytes and activated T lymphocytes. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In cats, this is referred to as the feline leukocyte antigen (FLA) region.
The complex retrovirus FIV (feline immunodeficiency virus), is responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency virus (HIV). The intensity of MHC Class II expression on cat lymphocyte membranes vary in association with feline retrovirus infections (Ohno et al. 1992).
The complex retrovirus FIV (feline immunodeficiency virus), is responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency virus (HIV). The intensity of MHC Class II expression on cat lymphocyte membranes vary in association with feline retrovirus infections (Ohno et al. 1992).
Product Details
- Target Species
- Cat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.05% Sodium Azide (NaN3) - Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | |||
| Immunofluorescence | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- MCA2724 detects band/s of approximately 29-31kDa on fractionated cat lymphocyte cell lysates under non-reducing conditions.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | 0.5 mg | F | ||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | 1 ml | F | ||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | 1 mg | C E P RE WB | ||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | 1 mg | F |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F |
Product Specific References
References for MHC Class II antibody
-
Zhang L et al. (2015) Phenotypic and functional analysis of CD1a+ dendritic cells from cats chronically infected with feline immunodeficiency virus.
Comp Immunol Microbiol Infect Dis. pii: S0147-9571(15)00055-7. -
Litster, A.L. et al. (2013) Diagnostic utility of a direct immunofluorescence test to detect feline coronavirus antigen in macrophages in effusive feline infectious peritonitis.
Vet J. 198 (2): 362-6.
Further Reading
-
Hunt, P. et al. (1995) Variable expression of major histocompatibility complex class II in the domestic cat.
Res Vet Sci. 59 (3): 195-200.
