Mouse anti Cat MHC class II antibody, clone PF8J-9B
recognizes an epitope within the ~30 kDa beta chain region of cat MHC class II molecule, expressed by antigen presenting cells, B cells, monocytes and activated T lymphocytes. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In cats, this is referred to as the feline leukocyte antigen (FLA) region.
The complex retrovirus FIV (feline immunodeficiency virus), is responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency virus (HIV). The intensity of MHC Class II expression on cat lymphocyte membranes vary in association with feline retrovirus infections (Ohno et al. 1992
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MHC Class II antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- MCA2724 detects band/s of approximately 29-31kDa on fractionated cat lymphocyte cell lysates under non-reducing conditions.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for MHC Class II antibody
Zhang L et al. (2015) Phenotypic and functional analysis of CD1a+ dendritic cells from cats chronically infected with feline immunodeficiency virus.
Comp Immunol Microbiol Infect Dis. pii: S0147-9571(15)00055-7.
Litster, A.L. et al. (2013) Diagnostic utility of a direct immunofluorescence test to detect feline coronavirus antigen in macrophages in effusive feline infectious peritonitis.
Vet J. 198 (2): 362-6.
Simões, R.D. et al. (2019) Effects of Regulatory T Cell Depletion on NK Cell Responses against Listeria monocytogenes in Feline Immunodeficiency Virus Infected Cats.
Viruses. 11 (11)Oct 24 [Epub ahead of print].
Hunt, P. et al. (1995) Variable expression of major histocompatibility complex class II in the domestic cat.
Res Vet Sci. 59 (3): 195-200.
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