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CD8 Alpha antibody | FE1.10E9

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Mouse anti Cat CD8 Alpha

Product Type
Monoclonal Antibody
Clone
FE1.10E9
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2052
Datasheet
F
MSDS
2 ml loader

Mouse anti Cat CD8 alpha antibody, clone FE1.10E9 recognizes the feline homolog of CD8 alpha and detects a broad band between 28-32 kDa by immunoprecipitation (Woo et al. 1997).

CD8 exists as an alpha/beta heterodimer and is expressed on a subpopulation of T lymphocytes.

Product Details

Target Species
Cat
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P41688
Entrez Gene
CD8A
GO Terms
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of CD8 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for CD8 Alpha antibody

  1. Woo, J.C. & Moore, P.F. (1997) A feline homologue of CD1 is defined using a feline-specific monoclonal antibody.
    Tissue Antigens. 49 (3 Pt 1): 244-51.
  2. Woo, J.C. et al. (1997) Immunopathologic changes in the thymus during the acute stage of experimentally induced feline immunodeficiency virus infection in juvenile cats.
    J Virol. 71 (11): 8632-41.
  3. Stein, V.M. et al. (2012) Miglustat improves purkinje cell survival and alters microglial phenotype in feline Niemann-Pick disease type C.
    J Neuropathol Exp Neurol. 71 (5): 434-48.
  4. Martini, V. et al. (2018) Flow cytometry for feline lymphoma: a retrospective study regarding pre-analytical factors possibly affecting the quality of samples.
    J Feline Med Surg. 20 (6): 494-501.
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