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CD5 antibody | FE1.1B11

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Anti Cat CD5 Antibody, clone FE1.1B11 thumbnail image 1
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Published customer image:
Mouse anti Cat CD5 antibody, clone FE1.1B11 (MCA2038S) used for the immunophenotyping of feline lymphoma samples by flow cytometry.
Image caption:
Representative plots of immunophenotyping by flow cytometry. (A) FNA of an intestinal mass, alimentary B‐cell lymphoma, CD4− (0%), CD5− (0%), CD8α/β− (0%), CD21+ (95%) (cat #1). (B) FNA of a peripheral lymph node, multicentric lymphoma, CD4+ (31%), CD5+ (53%), CD8α/β+ (15%), CD21+ (32%) (failure in detection of a predominant antigenically homogeneous lymphoid population) (cat #10). (C) FNA of a peripheral lymph node, reactive lymphoid hyperplasia, CD4+ (21%), CD5+ (53%), CD8α/β+ (26%), CD21+ (44%) (cat #16). *Red line (isotype control), green line (test sample).

From: Guzera M, Cian F, Leo C, Winnicka A, Archer J.
The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
Vet Comp Oncol. 2016 Aug;14 Suppl 1:40-51.
doi: 10.1111/vco.12098.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Cat CD5:RPE

Product Type
Monoclonal Antibody
Clone
FE1.1B11
Isotype
IgG1
Specificity
CD5

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Product Code Applications Pack Size List Price Your Price Qty
MCA2038PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
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Mouse anti Cat CD5 antibody, clone FE1.1B11 recognizes feline CD5, a 67kDa cell surface glycoprotein expressed by peripheral T lymphocytes and thymocytes.

Target Species
Cat
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
This product should be stored undiluted.
DO NOT FREEZE.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

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References for CD5 antibody

  1. Roccabianca, P. et al. (2000) Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine.
    Vet Immunol Immunopathol. 75 (1-2): 27-42.
  2. Riondato, F. et al. (2003) Effects of interferon alpha (INF-alpha) therapy on peripheral blood lymphocyte subsets from FIV and FeLV naturally infected cats.
    Vet Res Commun. 27: 429-32.
  3. Affolter, V.K. and Moore, P.F. (2006) Feline progressive histiocytosis.
    Vet Pathol. 43: 646-55.
  4. Roccabianca, P. et al. (2006) Feline large granular lymphocyte (LGL) lymphoma with secondary leukemia: primary intestinal origin with predominance of a CD3/CD8(alpha)(alpha) phenotype.
    Vet Pathol. 43: 15-28.
  5. Abkowitz, J.L. et al. (2009) In utero transplantation of monocytic cells in cats with alpha-mannosidosis.
    Transplantation. 88: 323-9.
  6. Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
    Vet Comp Oncol. 14 Suppl 1: 40-51.
  7. Rütgen, B.C. et al. (2022) Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats.
    J Feline Med Surg. 24 (2): 77-90.
  8. Cha, S. et al. (2023) Non-B, Non-T Acute Lymphoblastic Leukemia in a Cat
    Journal of Veterinary Clinics. 40 (4): 298-302.
    9. View The Latest Product References

RRID
AB_324002

MCA2038PE

INN1710

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