CD134 antibody | 7D6
lane 1 - Feline CD134 expressing cells
lane 2 - Control cells
lane 3 - Recombinant feline CD134-Fc fusion protein.
Mouse anti Cat CD134
- Product Type
- Monoclonal Antibody
- Clone
- 7D6
- Isotype
- IgG1
- Specificity
- CD134
Antibody Quality and Performance Guarantee
| Mouse anti Cat CD134 antibody, clone 7D6 recognizes feline CD134, otherwise known as OX40, a 43 kDa type I membrane protein and member of the tumor necrosis factor receptor superfamily, expressed predominantly by CD4+ activated T cells, and a key regulator of T cell-dependent immune responses. CD134 has been identified as the receptor for the OX40 ligand, CD252, expressed by activated B cells, acting as a co-stimulatory signal for the stimulation and secretion of immunoglobulins. CD134 has also been identified as a binding receptor for Feline Immunodeficiency Virus (FIV), acting along with CXC chemokine receptor 4 (CXCR4) to facilitate the entry of the virus into CD4+ primary target cells. |
- Target Species
- Cat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Weak Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- CHO-derived feline CD134-Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the NS0 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Western Blotting | |
1.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- MCA2568GA detects a band of approximately 43kDa.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rabbit F(ab')2 anti Mouse IgG:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79.
References for CD134 antibody
-
Willett, B.J. et al. (2009) Enforced covalent trimerisation of soluble feline CD134 (OX40)-ligand generates a functional antagonist of feline immunodeficiency virus.
Mol Immunol. 46: 1020-30. -
McDonnel, S.J. et al. (2012) Pharmacologic reactivation of latent feline immunodeficiency virus ex vivo in peripheral CD4+ T-lymphocytes.
Virus Res. 170 (1-2): 174-9.
Further Reading
-
Stüber, E. & Strober, W. (1996) The T cell-B cell interaction via OX40-OX40L is necessary for the T cell-dependent humoral immune response.
J Exp Med. 183 (3): 979-89. -
de Parseval, A. et al. (2004) Feline immunodeficiency virus targets activated CD4+ T cells by using CD134 as a binding receptor.
Proc Natl Acad Sci U S A. 101 (35): 13044-9.
- RRID
- AB_808380
MCA2568GA
0714
151615
230714
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD134 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Cat ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up