CD134 antibody | 7D6

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MCA2568GA
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SDS Safety Datasheet SDS
F WB 0.1 mg loader
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Mouse anti Cat CD134 antibody, clone 7D6 recognizes feline CD134, otherwise known as OX40, a 43 kDa type I membrane protein and member of the tumor necrosis factor receptor superfamily, expressed predominantly by CD4+ activated T cells, and a key regulator of T cell-dependent immune responses.

CD134 has been identified as the receptor for the OX40 ligand, CD252, expressed by activated B cells, acting as a co-stimulatory signal for the stimulation and secretion of immunoglobulins. CD134 has also been identified as a binding receptor for Feline Immunodeficiency Virus (FIV), acting along with CXC chemokine receptor 4 (CXCR4) to facilitate the entry of the virus into CD4+ primary target cells.

Target Species
Cat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Weak
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
CHO-derived feline CD134-Fc fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blotting
MCA2568GA detects a band of approximately 43kDa.

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References for CD134 antibody

  1. Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
    J Virol. 81: 9665-79.
  2. Willett, B.J. et al. (2009) Enforced covalent trimerisation of soluble feline CD134 (OX40)-ligand generates a functional antagonist of feline immunodeficiency virus.
    Mol Immunol. 46: 1020-30.
  3. McDonnel, S.J. et al. (2012) Pharmacologic reactivation of latent feline immunodeficiency virus ex vivo in peripheral CD4+ T-lymphocytes.
    Virus Res. 170 (1-2): 174-9.

Further Reading

  1. Stüber, E. & Strober, W. (1996) The T cell-B cell interaction via OX40-OX40L is necessary for the T cell-dependent humoral immune response.
    J Exp Med. 183 (3): 979-89.
  2. de Parseval, A. et al. (2004) Feline immunodeficiency virus targets activated CD4+ T cells by using CD134 as a binding receptor.
    Proc Natl Acad Sci U S A. 101 (35): 13044-9.

Flow Cytometry

RRID
AB_808380
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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