Canakinumab antibody | AbD54142
Human anti Canakinumab (Drug/Target Complex)
- Product Type
- Monoclonal Antibody
- Clone
- AbD54142
- Isotype
- Fab antibody
- Specificity
- Canakinumab
- Region
- DRUG/TARGET COMPLEX
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, the IL-1 beta/canakinumab complex was formed by adding 5 μg/ml canakinumab to wells coated in IL-1 beta. Detection was performed using HRP conjugated Human Anti-Canakinumab Antibody, clone AbD54142pap (TZA0112P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with canakinumab, IL-1 beta, human IgG1 kappa control and Trastuzumab at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, the IL-1 beta/canakinumab complex was formed by adding 5 µg/mL canakinumab to the wells coated with IL-1 beta. Detection was performed using Human Anti-Canakinumab (Drug/Target Complex) Antibody, clone AbD54142ad (TZA0112) titrated to the given concentrations followed by an HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for IL-1 beta/canakinumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with IL-1 beta at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of canakinumab. Detection was performed using HRP-conjugated Human Anti-Canakinumab (Drug/Target Complex) Antibody, clone AbD54142pap (TZA0112P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A), and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
| Human Anti-Canakinumab (Drug/Target Complex) Antibody, clone AbD54142 specifically recognizes the antibody drug canakinumab when in complex with its target, the human interleukin-1 beta (IL-1β). It does not recognize free canakinumab nor unbound IL-1β. The monovalent Fab format of this antibody, clone AbD54142ad (TZA0112) is tagged with a SpyTag2 at the C-terminus of the Fab heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour. This antibody is also available as a bivalent Fab antibody pre-coupled to the HRP-conjugated BiSpyCatcher2 (TZC002P) under product code TZA0112P for direct detection of the Canakinumab/ IL-1β complex in bioanalysis assays. Clone AbD54142 can be used in pharmacokinetic (PK) antigen capture format assays to measure canakinumab that has been captured via immobilized IL-1β. It is ideal for bioanalytical assays to measure the level of drug or biosimilar in complex with the IL-1β target. Canakinumab (trade name Ilaris) is a fully humanized monoclonal IgG1/kappa antibody with allotype G1m3. It binds to interleukin-1 beta (IL-1β) to neutralize up-regulated 1β signaling, resulting in suppression of inflammation in patients with disorders of autoimmune origin. Canakinumab is indicated for treatment of Cryopyrin-Associated Periodic Syndromes (CAPS), three rare and serious auto-inflammatory diseases, Still’s disease, and gouty arthritis. View a summary of all anti-canakinumab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Canakinumab/IL-1 beta complex
- Affinity
- The monovalent intrinsic affinity of AbD54142ad was measured as KD = 3.3 nM by real time, label free molecular interaction analysis on immobilized human IL-1 beta/canakinumab complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Ilaris is a trademark of Novartis AG. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD54142ad may be used in an indirect ELISA to detect the human IL-1 beta/canakinumab complex or to detect canakinumab bound to immobilized IL-1 beta.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Canakinumab | HCA417 | E | 0.1 mg |
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| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab | HCA418 | E | 0.1 mg |
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| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab | HCA419 | E | 0.1 mg |
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| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab (Drug/Target Complex):HRP | TZA0112P | E | 0.1 mg |
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| Description | Human anti Canakinumab (Drug/Target Complex):HRP | ||||||
| Human anti Canakinumab | TZA063 | E | 0.1 mg |
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| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab | TZA064 | E | 0.1 mg |
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| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab:HRP | TZA064P | E | 0.1 mg |
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| Description | Human anti Canakinumab:HRP | ||||||
- Synonyms
- Ilaris
TZA0112
171092
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Canakinumab specificity
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