Canakinumab antibody | AbD54142
Human anti Canakinumab (Drug/Target Complex):HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD54142
- Isotype
- Fab antibody
- Specificity
- Canakinumab
- Region
- DRUG/TARGET COMPLEX
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, the IL-1 beta/canakinumab complex was formed by adding 5 μg/ml canakinumab to wells coated in IL-1 beta. Detection was performed using HRP conjugated Human Anti-Canakinumab Antibody, clone AbD54142pap (TZA0112P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with canakinumab, IL-1 beta, human IgG1 kappa control and Trastuzumab at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, the IL-1 beta/canakinumab complex was formed by adding 5 µg/mL canakinumab to the wells coated with IL-1 beta. Detection was performed using Human Anti-Canakinumab (Drug/Target Complex) Antibody, clone AbD54142ad (TZA0112) titrated to the given concentrations followed by an HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for IL-1 beta/canakinumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with IL-1 beta at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of canakinumab. Detection was performed using HRP-conjugated Human Anti-Canakinumab (Drug/Target Complex) Antibody, clone AbD54142pap (TZA0112P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A), and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
| Human Anti-Canakinumab (Drug/Target Complex) Antibody, clone AbD54142 specifically recognizes the antibody drug canakinumab when in complex with its target, the human interleukin-1 beta (IL-1β). It does not recognize free canakinumab nor unbound IL-1β. The monovalent Fab format of this antibody, clone AbD54142ad (TZA0112) is tagged with a SpyTag2 at the C-terminus of the Fab heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour. This antibody is also available as a bivalent Fab antibody pre-coupled to the HRP-conjugated BiSpyCatcher2 (TZC002P) under product code TZA0112P for direct detection of the Canakinumab/ IL-1β complex in bioanalysis assays. Clone AbD54142 can be used in pharmacokinetic (PK) antigen capture format assays to measure canakinumab that has been captured via immobilized IL-1β. It is ideal for bioanalytical assays to measure the level of drug or biosimilar in complex with the IL-1β target. Canakinumab (trade name Ilaris) is a fully humanized monoclonal IgG1/kappa antibody with allotype G1m3. It binds to interleukin-1 beta (IL-1β) to neutralize up-regulated 1β signaling, resulting in suppression of inflammation in patients with disorders of autoimmune origin. Canakinumab is indicated for treatment of Cryopyrin-Associated Periodic Syndromes (CAPS), three rare and serious auto-inflammatory diseases, Still’s disease, and gouty arthritis. View a summary of all anti-canakinumab antibodies |
- Product Form
- A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA0112 with a HRP conjugated BiSpyCatcher version 2 (TZC002P). The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Canakinumab/IL-1 beta complex
- Affinity
- The monovalent intrinsic affinity of AbD54142pap was measured as KD = 3.3 nM by real time, label free molecular interaction analysis on immobilized human IL-1 beta/canakinumab complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Ilaris is a trademark of Novartis AG. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD54142pap may be used in a direct ELISA to detect the human IL-1 beta/canakinumab complex or as a detection reagent for canakinumab captured via immobilised IL-1 beta.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Hispec Assay Diluent | ||||||
| Human anti Canakinumab | HCA417 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab | HCA418 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab | HCA419 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab (Drug/Target Complex) | TZA0112 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Canakinumab (Drug/Target Complex) | ||||||
| Human anti Canakinumab | TZA063 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab | TZA064 | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Canakinumab | ||||||
| Human anti Canakinumab:HRP | TZA064P | E | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Human anti Canakinumab:HRP | ||||||
- Synonyms
- Ilaris
TZA0112P
171093
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Canakinumab specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up
