Brentuximab Vedotin antibody | AbD39659ia

Human anti Brentuximab Vedotin

Product Type
Monoclonal Antibody
Clone
AbD39659ia
Isotype
IgG1
Specificity
Brentuximab Vedotin

Product Code Applications Pack Size List Price Your Price Qty
HCA352
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Human Anti-Brentuximab vedotin Antibody, clone AbD39659ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug brentuximab, both alone and as the antibody drug conjugate (ADC), brentuximab vedotin.

Clone AbD39659ia is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 1.4 nM and is one of three antibodies with different affinities offered for ADA assays. Antibody HCA350 has affinity 6 nM and HCA351 has affinity 0.7 nM.

Brentuximab vedotin (Adcetris) is a chimeric mouse/human IgG1 kappa ADC, approved by the FDA for the treatment of Hodgkin’s lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). The brentuximab antibody selectively targets tumor cells expressing the cell membrane protein CD30, and delivers the conjugated toxin, monomethyl auristatin E (MMAE). Once inside the cell the MMAE is released from the antibody by proteolytic cleavage and disrupts the microtubule network inducing cell cycle arrest and apoptosis of the malignant cells, thereby preventing tumor growth and proliferation.

View a summary of all brentuximab vedotin antibodies

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Brentuximab vedotin
Affinity
The intrinsic affinity of the monovalent form of Human anti Brentuximab Vedotin antibody, clone AbD39659ia is KD = 1.4 nM as measured by real time, label free molecular interaction analysis on immobilized Brentuximab Vedotin.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Adcentris is a trademark of Seattle Genetics.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
Human anti Brentuximab Vedotin antibody, clone AbD39659ia can be used as a surrogate positive control or calibrator in an anti-drug antibody assay.

Protocol: ADA bridging ELISA.

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LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Brentuximab Vedotin HCA349 E 0.1 mg loader
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Description Human anti Brentuximab Vedotin
Human anti Brentuximab Vedotin HCA350 E 0.1 mg loader
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Description Human anti Brentuximab Vedotin
Human anti Brentuximab Vedotin HCA351 E 0.1 mg loader
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Description Human anti Brentuximab Vedotin

Synonyms
Adcetris
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"