Benralizumab antibody | AbD59979
Human anti Benralizumab (Drug/Target Complex)
- Product Type
- Monoclonal Antibody
- Clone
- AbD59979
- Isotype
- Fab antibody
- Specificity
- Benralizumab
- Region
- DRUG/TARGET COMPLEX
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Benralizumab Antibody, clone AbD59979oap (TZA097P) at a concentration of 2 μg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with benralizumab, IL5Ra-His, and human IgG1 kappa control at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, the IL5Ra-His/benralizumab complex was formed by adding 5 µg/mL benralizumab to the wells coated with 5 µg/mL IL5Ra-His. Detection was performed using Human Anti-Benralizumab (Drug/Target Complex) Antibody, clone AbD59979ad (TZA097) titrated to the given concentrations followed by an HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for IL5Ra-His/benralizumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with IL5Ra-His at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of benralizumab. Detection was performed using HRP-conjugated Human Anti-Benralizumab (Drug/Target Complex) Antibody, clone AbD59979oap (TZA097P) at a concentration of 0.2 µg/mL in HISPEC Assay Diluent (BUF049A), and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-Benralizumab (Drug/Target Complex) Antibody, clone AbD59979 specifically recognizes the antibody drug benralizumab when in complex with its target, human interleukin 5 receptor α (IL5Rα ). It does not recognize free benralizumab nor unbound IL-5R. The monovalent Fab format of this antibody, clone AbD59979ad (TZA097) is tagged with a SpyTag2 at the C-terminus of the Fab heavy chain, enabling the user to couple it to a SpyCatcher reagent for conversion to alternative formats in less than one hour. This antibody is also available as a monovalent Fab antibody pre-coupled to the HRP-conjugated SpyCatcher2 (TZC002P) under product code TZA097P for direct detection of the benralizumab/ IL5Rα complex in bioanalysis assays. Clone AbD59979 can be used in pharmacokinetic (PK) antigen detection format assays to measure benralizumab that has been captured via immobilized IL5Rα. Benralizumab (trade name Fasenra) is a humanized monoclonal antibody of the IgG1/kappa isotype that binds with high affinity to a conformationally distinct epitope within domain 1 of the α-chain of IL-5R, and thus inhibits proliferation of IL-5-dependent cell lines, and otherwise blocks signaling through the IL-5 receptor. Benralizumab is indicated as a maintenance treatment for severe asthma and an eosinophilic phenotype. View a summary of all anti-benralizumab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Benralizumab/IL5Rα complex
- Affinity
- The monovalent intrinsic affinity of AbD59979ad was measured as KD = 1 nM by real time, label free molecular interaction analysis on immobilized human IL5Rα/benralizumab complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Fasenra is a trademark of MedImmune, now owned by AstraZeneca. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD59979ad may be used in an indirect ELISA to detect the human IL5Rα/benralizumab complex or to detect benralizumab bound to immobilized human IL5Rα.
Protocol: PK antigen capture ELISA
- Synonyms
- Fasenra
TZA097
171075
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Benralizumab specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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