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Atezolizumab antibody | AbD40992ia

Human anti Atezolizumab

Product Type
Monoclonal Antibody
Clone
AbD40992ia
Isotype
IgG1
Specificity
Atezolizumab

Product Code Applications Pack Size List Price Your Price Qty
HCA381
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Atezolizumab Antibody, clone AbD40992ia is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free monoclonal antibody drug atezolizumab. It does not recognize the drug target, Programmed Cell Death Ligand 1 (PD-L1), nor atezolizumab in complex with PD-L1. The antibody can be used to measure levels of free atezolizumab in patient samples.

Clone AbD40992ia is a fully human recombinant monoclonal antibody with IgG1 isotype. It can be used in a direct or indirect ELISA, or as a detection reagent for atezolizumab in a PK bridging ELISA together with TZA011 (AbD40638ad) as the capture reagent, or to develop and calibrate anti drug antibody (ADA) assays to measure the ADA response in patient sera together with HCA380 and HCA382.This antibody has a monovalent intrinsic affinity of 1.4 nM.

Atezolizumab (Tecentriq) is a human monoclonal IgG1 antibody that binds directly to Programmed Death Ligand 1 (PD-L1, CD274) and provides a dual blockade of both the PD-1 and B7.1 receptors on T-cells and antigen presenting cells. It is one of the named “checkpoint inhibitor” drugs. PD-L1/PD-1 mediated inhibition of the immune response is thereby released, including reactivation of the anti-tumour immune response without induction of antibody-dependent cellular cytotoxicity. The drug is used to treat urothelial carcinoma, non-small cell lung cancer, triple-negative breast cancer, small cell lung cancer and hepatocellular carcinoma. It is used either on its own or in combination for cancers which are advanced or metastatic.

View a summary of all anti-atezolizumab antibodies

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Atezolizumab
Affinity
The intrinsic affinity of the monovalent form of Human anti Atezolizumab antibody, clone AbD40992ia is KD = 1.4 nM as measured by real time, label free molecular interaction analysis on immobilized Atezolizumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Tecentriq is a trademark of Roche/ Genentech.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used in a direct or indirect ELISA or as a detection reagent for atezolizumab in a PK bridging ELISA together with TZA011 (AbD40638ad) as the capture reagent.

Protocol: PK bridging ELISA

It can also be used to develop and calibrate anti drug antibody (ADA) assays to measure the ADA response in patient sera together with HCA380 and HCA382

Protocol: ADA bridging ELISA.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Atezolizumab HCA380 E 0.1 mg loader
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loader
Description Human anti Atezolizumab
Human anti Atezolizumab HCA382 E 0.1 mg loader
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loader
Description Human anti Atezolizumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Human anti Atezolizumab TZA011 E 0.1 mg
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Description Human anti Atezolizumab

Synonyms
Tecentriq
RRID
AB_2943068
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA381

166025

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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