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Atezolizumab antibody | AbD40638ia

Human anti Atezolizumab

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
New HCA382
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg
List Price Your Price

Human Anti-Atezolizumab Antibody, clone AbD40638ia is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free monoclonal antibody drug atezolizumab. This is a humanized, Fc-engineered IgG1/kappa antibody with allotype nG1m1. The CH2 domain was engineered with one amino acid substitution (N297A) resulting in a non-glycosylated antibody, to impair Fcγ receptor binding and to prevent Fc-mediated depletion of cells expressing PD-L1.The antibody does not recognize free PD-L1 nor atezolizumab in complex with PD-L1.

Clone AbD40638ia is a fully human recombinant monoclonal antibody with IgG1 isotype and can be used as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 0.4 nM, and is one of three Type 1 antibodies with different affinities offered for ADA assays.

ADA bridging ELISA

Clone AbD40638ia is also available in a monovalent Fab format with a SpyTag as TZA011.

Atezolizumab (Tecentriq) is a human monoclonal IgG1 antibody that binds directly to Programmed Death Ligand 1 (PD-L1, CD274) and provides a dual blockade of both the PD-1 and B7.1 receptors on T-cells and antigen presenting cells. It is one of the named “checkpoint inhibitor” drugs. PD-L1/PD-1 mediated inhibition of the immune response is thereby released, including reactivation of the anti-tumour immune response without induction of antibody-dependent cellular cytotoxicity. The drug is used to treat urothelial carcinoma, non-small cell lung cancer, triple-negative breast cancer, small cell lung cancer and hepatocellular carcinoma. It is used either on its own or in combination for cancers which are advanced or metastatic.

View a summary of all anti-atezolizumab antibodies

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
The intrinsic affinity of the monovalent form of Human anti Atezolizumab antibody, clone AbD40638ia is KD = 0.4 nM as measured by real time, label free molecular interaction analysis on immobilized Atezolizumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
Tecentriq is a trademark of Roche/ Genentech..

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Human anti Atezolizumab antibody, clone AbD40638ia can be used in a direct and indirect ELISA. It can also be used as a calibrator in an anti-drug (ADA) antibody assay.

Protocol: ADA bridging ELISA.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Atezolizumab HCA380 E 0.1 mg
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Description Human anti Atezolizumab
Human anti Atezolizumab HCA381 E 0.1 mg
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Description Human anti Atezolizumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
Human anti Atezolizumab TZA011 E 0.1 mg
List Price Your Price
Description Human anti Atezolizumab

Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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