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PFKP antibody | OTI1F2

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KO Validated
IP-MS Validated
Anti PFKP Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti PFKP Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti PFKP Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti PFKP Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti PFKP Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of PFKP knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti PFKP antibody (VMA00145) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 95 second and (B) 30 second exposure.
Anti PFKP Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) gallery image 2
Normalized signal intensity of PFKP from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti PFKP Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates probed with Mouse anti Human PFKP antibody (VMA00145) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 15 second exposure. Arrow points to PFKP (molecular weight 86 kDa).
Anti PFKP Antibody, clone OTI1F2 (PrecisionAb Monoclonal Antibody) gallery image 4
Immunoprecipitation of PFKP from Jurkat whole cell lysate. The Input lane is 10% input; the PFKP-IP lane is the immunoprecipitation with the PFKP antibody (2.5 µg, VMA00145); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using PFKP antibody (1/1,000, VMA00145) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to PFKP.

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WB IP

Mouse anti PFKP

Product Type
PrecisionAb Monoclonal
Clone
OTI1F2
Isotype
IgG1
Format
Purified
Specificity
PFKP

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VMA00145
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti Human PFKP antibody recognizes ATP-dependent 6-phosphofructokinase, platelet type (PFKP), also known as 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type, phosphofructokinase, platelet type, phosphofructo-1-kinase isozyme C, phosphofructokinase 1 and phosphohexokinase.

PFKP catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. The muscle and liver isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2 (Vora 1981).

Mouse anti Human PFKP antibody detects a band of 86 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PFKP detects a band of approximately 86 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human PFKP (NP_002618) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

Further Reading

  1. Vora S (1981) Isozymes of human phosphofructokinase in blood cells and cultured cell lines: molecular and genetic evidence for a trigenic system.
    Blood. 57 (4): 724-32.

UniProt
Q01813
Entrez Gene
PFKP
GO Terms
GO:0005515 protein binding
GO:0003872 6-phosphofructokinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005945 6-phosphofructokinase complex
GO:0006096 glycolysis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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