PFKP antibody | OTI1F2

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Mouse anti PFKP

PFKP antibody recognizes 6-phosphofructokinase type C, also known as 6-phosphofructokinase, platelet type, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type, phosphofructo-1-kinase isozyme C or phosphohexokinase.

PFKP Antibody with Control Lysate

PFKP antibody recognizes 6-phosphofructokinase type C, also known as 6-phosphofructokinase, platelet type, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type, phosphofructo-1-kinase isozyme C or phosphohexokinase.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1F2
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00145 WB 100 µl
VMA00145KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PFKP antibody recognizes ATP-dependent 6-phosphofructokinase, platelet type (PFKP), also known as 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type, phosphofructokinase, platelet type, phosphofructo-1-kinase isozyme C, phosphofructokinase 1 and phosphohexokinase.

PFKP catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. The muscle and liver isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.

Mouse anti Human PFKP antibody detects a band of 86 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PFKP detects a band of approximately 86 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human PFKP (NP_002618) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q01813 Related reagents
Entrez Gene
PFKP Related reagents
GO Terms
GO:0005515 protein binding
GO:0003872 6-phosphofructokinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005945 6-phosphofructokinase complex
GO:0006096 glycolysis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PFKP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Product Specific References

Further Reading

  1. Vora S (1981) Isozymes of human phosphofructokinase in blood cells and cultured cell lines: molecular and genetic evidence for a trigenic system.
    Blood. 57 (4): 724-32.

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