PFKP antibody | OTI1F2

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Anti PFKP Antibody, clone OTI1F2 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PFKP antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 15 second exposure. Arrow points to PFKP (molecular weight 86 kDa)

Mouse anti PFKP

PFKP antibody recognizes 6-phosphofructokinase type C, also known as 6-phosphofructokinase, platelet type, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type, phosphofructo-1-kinase isozyme C or phosphohexokinase.

PFKP Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1F2
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00145	WB	100 µl
VMA00145KT	WB	2 Westerns
With Lysate		400µg

Mouse anti Human PFKP antibody recognizes ATP-dependent 6-phosphofructokinase, platelet type (PFKP), also known as 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type, phosphofructokinase, platelet type, phosphofructo-1-kinase isozyme C, phosphofructokinase 1 and phosphohexokinase.

PFKP catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. The muscle and liver isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2 (Vora 1981).

Mouse anti Human PFKP antibody detects a band of 86 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti PFKP detects a band of approximately 86 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human PFKP (NP_002618) produced in HEK293T cells
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q01813 Related reagents
Entrez Gene: PFKP Related reagents
GO Terms: GO:0005515 protein binding; GO:0003872 6-phosphofructokinase activity; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005945 6-phosphofructokinase complex; GO:0006096 glycolysis
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of PFKP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

Product Specific References

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