TrailBlazer StarBright Violet 515 Label Kit
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CJ ResetTrailBlazer SBV515 Label Kit 3x 0.1 mg
- Product Type
- Conjugation Kit
- Specificity
- TrailBlazer StarBright Violet 515 Label Kit
Antibody Quality and Performance Guarantee| Product Code | Applications | Pack Size | List Price | Your Price | Qty | ||||||||||
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17010409
TrailBlazerTM StarBrightTM Dye Label is the second part of a two-kit antibody labeling system. Together with the TrailBlazer Tag kit it will allow you to conjugate an antibody to superior StarBright Dyes using the innovative SpyTag and SpyCatcher technology. They provide the benefits of using StarBright Dyes in flow cytometry and western blots for your antibody of choice. First, the SpyTag is attached to an antibody using the TrailBlazer Tag Kit. This antibody, now with a SpyTag, is then mixed with a StarBright Dye labeled SpyCatcher using the Label Kit. A spontaneous reaction occurs between the SpyTag and SpyCatcher resulting in an irreversible covalent isopeptide bond. The result is a highly stable StarBright Dye labeled antibody, which should be stored at +4°C in the dark, and when stored correctly will be stable for up to 12 months. Batch specific Certificate of Analysis for TrailBlazer StarBright Dye Label Kits are available to be downloaded directly from the website. In flow cytometry we suggest using 5µl of the undiluted labeled antibody to stain 106 cells in 100µl. However, we highly recommend that you titrate the antibody to determine the optimal amount of antibody for your specific experiment. The TrailBlazer StarBright Dye Label Kits are available in different pack sizes and for a range of StarBright Dyes. Discover the full range and all the benefits of these TrailBlazer Tag and StarBright Dye Label Kits. |
- Reagents In The Kit
- 3 X 100 µg Antibody Conjugations (1 X 100 µg Antibody Conjugation Kit available)
3 vials Storage Buffer
3 vials StarBright Dye-Catcher SBV515
3 vials Quenching Reagent
3 Spin Columns
3 Collection Tubes - Max Ex/Em
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Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 515 402 516 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details
The StarBright Dye labeled antibody should be stored undiluted at +4°C in the dark for up to 1 year.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Conjugation |  |
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
- Instructions For Use
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17010409
- Add the SpyTagged antibody product from TrailBlazer Tag Kit to the StarBright Dye-Catcher vial. Mix and incubate for 30 min at room temperature or overnight at +4°C.
- Add the sample to the Spin Column. Vortex briefly and mix on an end-over-end rotary mixer at room temperature for 60 min.
- Break open the bottom of the Spin Column and insert the column into the 2 ml amber collection tube. Slightly loosen the cap of the Spin Column and centrifuge at 1500 x g for 2 min. Retain the eluate, containing the label antibody.
- Prepare Quenching Reagent by adding 5 µl of ultrapure water.
- Add 4 µl of prepared Quenching Reagent and 45 µl of Storage Buffer to the labeled antibody. Vortex briefly to mix.
Antibodies are now labeled and ready to use. Store at +4°C in the dark for up to 1 year.
For full instructions for use please go to: bio-rad-antibodies.com/label-SB-kit-IFU.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| TrailBlazer Tag Kit 3 X 0.1 mg | 12020901 | CJ | 3 X 100 µg Antibody Conjugations |
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| Description | TrailBlazer Tag Kit 3 X 0.1 mg | ||||||
| TrailBlazer SBV440 Label Kit 3x 0.1 mg | 17010258 | CJ | 3 X 100 µg Antibody Conjugations |
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| Description | TrailBlazer SBV440 Label Kit 3x 0.1 mg | ||||||
| TrailBlazer SBV570 Label Kit 3x 0.1 mg | 17010273 | CJ | 3 X 100 µg Antibody Conjugations |
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| List Price | Your Price | ||||||
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| Description | TrailBlazer SBV570 Label Kit 3x 0.1 mg | ||||||
| TrailBlazer SBB700 Label Kit 3x 0.1 mg | 17010362 | CJ | 3 X 100 µg Antibody Conjugations |
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| Description | TrailBlazer SBB700 Label Kit 3x 0.1 mg | ||||||
| TrailBlazer SBV610 Label Kit 3x 0.1 mg | 17010408 | CJ | 3 X 100 µg Antibody Conjugations |
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| List Price | Your Price | ||||||
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| Description | TrailBlazer SBV610 Label Kit 3x 0.1 mg | ||||||
| TrailBlazer SBV670 Label Kit 3x 0.1 mg | 17010412 | CJ | 3 X 100 µg Antibody Conjugations |
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| List Price | Your Price | ||||||
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| Description | TrailBlazer SBV670 Label Kit 3x 0.1 mg | ||||||
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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