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Antibody Purification Kit Protein G

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Proteus Protein G Mini Purification Kit

Proteus Protein G MIDI Purification Kit

Product Type
Purification Kit
Product Code Applications Pack Size List Price Quantity
PUR016
Datasheet
PP
SDS
16 Units

PUR012
Datasheet
PP
SDS
4 Units

Product Details

Preservative Stabilisers
Buffers contain 0.09% Sodium Azide (NaN3)
Resin contains 20% Ethanol
Preservative Stabilisers
Buffers contain 0.09% Sodium Azide (NaN3)
Resin contains 20% Ethanol
Reagents in the Kit
16 x Protein G Spin Column Plugs
16 x Spin Columns
32 x 2.2 ml Centrifuge Tubes
16 x 10 kDa MWCO Ultrafiltration Spinners
1 x 250 ml of 1 x Buffer G
1 x 125 ml of 1 x Buffer B2
1 x 30 ml of 1 x Buffer C
Plug Insertion Tool
Instructions
Reagents in the Kit
4 x Protein G Spin Column Plugs
4 x Spin Columns
8 x 50 ml Centrifuge Tubes
4 x 10 kDa MWCO Ultrafiltration Spinners
1 x 250 ml of 1 x Buffer G
1 x 125 ml of 1 x Buffer B2
1 x 30 ml of 1 x Buffer C
Plug Insertion Tool
Instructions

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
Storage
Store at +4oC. DO NOT FREEZE.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Regulatory
For research purposes only

Applications of Antibody Purification Kit Protein G

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Protein Purification
Protein Purification
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mycoplasma Removal Agent BUF035 TC 5 ml
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Mycoplasma Removal Agent BUF035 TC 5 ml
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Proteus Protein A MIDI Purification Kit PUR003 PP 4 Units
Rat Monoclonal Antibody Isotyping Test Kit RMT1 IS 10 Tests loader

Product Specific References

References for Antibody Purification Kit Protein G

  1. Abdelkrim, M.A. et al. (2012) Autoantibodies against cardiac β(1)-adrenoceptor do not affect the low-affinity state β(1)-adrenoceptor-mediated inotropy in rat cardiomyocytes.
    Can J Physiol Pharmacol. 90: 407-14.
  2. Soler, L. et al. (2011) Development and validation of a novel competitive ELISA for the detection of serum amyloid A in pigs.
    Vet J. 190: e7-11.
  3. Kimura, K. et al. (2012) Pyrroloquinoline quinone stimulates epithelial cell proliferation by activating epidermal growth factor receptor through redox cycling.
    Free Radic Biol Med. 53: 1239-51.
  4. Montaudon, E. et al. (2014) Cardiovascular effects of beta1 and beta3-adrenergic receptor autoantibodies in Lewis rat.
    [Article in French]
    Ann Cardiol Angeiol (Paris). 63: 128-34.
  5. Yuan, W. et al. (2015) Quantitative analysis of immunoglobulin subclasses and subclass specific glycosylation by LC-MS-MRM in liver disease.
    J Proteomics. 116: 24-33.
  6. Broecker, F. et al. (2016) Multivalent display of minimal Clostridium difficile glycan epitopes mimics antigenic properties of larger glycans.
    Nat Commun. 7: 11224.
  7. Montaudon E et al. (2015) Cardiac effects of long-term active immunization with the second extracellular loop of human β1- and/or β3-adrenoceptors in Lewis rats.
    Pharmacol Res. 100: 210-9.

Fluorescent Spectraviewer

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How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra
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