Antibody Purification Kit Protein A
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See Binding affinities for Protein A and G. Recommended Reading - Proteus Protein A Purification Handbook.
Product Details
- Preservative Stabilisers
Buffers contain 0.09% Sodium Azide (NaN3) Resin contains 0.01% Thiomersal - Preservative Stabilisers
Buffers contain 0.09% Sodium Azide (NaN3) Resin contains 0.01% Thiomersal - Reagents in the Kit
- 16 x Protein A Spin Column Plugs 16 x Spin Columns 32 x 2.2 ml Centrifuge Tubes 16 x 10 kDa MWCO Ultrafiltration Spinners 1 x 250 ml of 1 x Buffer A 1 x 125 ml of 1 x Buffer B1 1 x 125 ml of 1 x Buffer B2 1 x 30 ml of 1 x Buffer C Plug Insertion Tool Instructions
- Reagents in the Kit
- 4 x Protein A Spin Column Plugs
4 x Spin Columns
8 x 50 ml Centrifuge Tubes
4 x 10 kDa MWCO Ultrafiltration Spinners
1 x 250 ml of 1 x Buffer A
1 x 125 ml of 1 x Buffer B1
1 x 125 ml of 1 x Buffer B2
1 x 30 ml of 1 x Buffer C
Plug Insertion Tool
Instructions
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
- Storage
- Store at +4oC. DO NOT FREEZE.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of Antibody Purification Kit Protein A
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Protein Purification | |||
| Protein Purification |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mycoplasma Removal Agent | BUF035 | TC | 5 ml | ||
| Mouse Monoclonal Antibody Isotyping Test Kit | MMT1 | IS | 10 Tests |
|
|
| Proteus Protein G Mini Purification Kit | PUR016 | PP | 16 Units | ||
| Rat Monoclonal Antibody Isotyping Test Kit | RMT1 | IS | 10 Tests |
|
|
| Mycoplasma Removal Agent | BUF035 | TC | 5 ml | ||
| Mouse Monoclonal Antibody Isotyping Test Kit | MMT1 | IS | 10 Tests |
|
|
| Proteus Protein G MIDI Purification Kit | PUR012 | PP | 4 Units | ||
| Rat Monoclonal Antibody Isotyping Test Kit | RMT1 | IS | 10 Tests |
|
Product Specific References
References for Antibody Purification Kit Protein A
-
Golchin, M. et al. (2012) Latex agglutination test based on single-chain Fv recombinant antibody fragment.
Scand J Immunol. 75: 38-45. -
Yuan, W. et al. (2015) Quantitative analysis of immunoglobulin subclasses and subclass specific glycosylation by LC-MS-MRM in liver disease.
J Proteomics. 116: 24-33. -
Jansen, C.A. et al. (2016) Identification of an Activating Chicken Ig-like Receptor Recognizing Avian Influenza Viruses.
J Immunol. 197 (12): 4696-703. -
Raeisi, H. et al. (2019) Applying the pthA effector protein of Xanthomonas citri. subsp. citr.i for production of specific antibodies and its application for detection of infected plants.
J Plant Pathol. 102 (1): 79-87.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
