Tubulin Beta 4B Antibody (PrecisionAbTM Antibody)

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Tubulin Beta 4B Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with tubulin beta 4b antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 3 second exposure. Arrow points to tubulin beta 4b (molecular weight 53 kDa)

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  • Tubulin Beta 4B Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Tubulin Beta 4B Antibody with Control Lysate
  • Rabbit anti Tubulin Beta 4B
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    Rabbit anti-human tubulin beta 4B antibody recognizes tubulin beta-4B chain, also known as class IVb beta tubulin, tubulin beta-2 chain or TUBB4B. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00445WBdatasheet pdfdatasheet pdf100 µl
      VPA00445
      VPA00445KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00445KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human tubulin beta 4B antibody recognizes tubulin beta 4B chain, also known as class IVb beta tubulin, tubulin beta-2 chain or TUBB4B.

        Rabbit anti Human tubulin beta 4B chain antibody detects a band of 53 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti tubulin beta 4B detects a band of approximately 53 kDa in C6 cell lysates
        Anti tubulin beta 4B detects a band of approximately 53 kDa in C6 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Rabbit polyclonal antibody purified by affinity chromatography
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH conjugated synthetic peptide corresponding to amino acid 99-125 of human tubulin beta 4B
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg C6 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        GTPase activity
        microtubule-based movement
        G2/M transition of mitotic cell cycle
        MHC class I protein binding
        structural molecule activity
        cytosol
        GTP binding
        natural killer cell mediated cytotoxicity
        microtubule
        'de novo' posttranslational protein folding
        unfolded protein binding
        protein polymerization
        cellular component movement
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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